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一种从大肠杆菌细胞中分离出的因子对翻译起始的抑制作用。

Inhibition of the initiation of translation by a factor isolated from Escherichia coli cells.

作者信息

Clark V L

出版信息

Proc Natl Acad Sci U S A. 1980 Feb;77(2):1181-4. doi: 10.1073/pnas.77.2.1181.

DOI:10.1073/pnas.77.2.1181
PMID:6987666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348449/
Abstract

A low molecular weight factor isolated from Escherichia coli cells was found to inhibit protein synthesis in vitro directed by RNA of bacteriophage R-17. The factor also inhibited poly(U)-directed translation, but higher concentrations were required. When the factor was added after R-17 RNA-directed translation was initiated, the onset of inhibition was delayed. Initiation-independent translation of polysomes was not inhibited. The factor inhibited the binding of N-formylmethionyl-tRNAfMet to 70S ribosomes to form the 70S initiation complex, and it released the N-flrmylmethionyl-tRNAfMet from preformed complexes. The factor did not prevent the formation of N-formylmethionylpuromycin. It was concluded that the factor inhibits specifically the initiation of translation.

摘要

从大肠杆菌细胞中分离出的一种低分子量因子被发现可在体外抑制由噬菌体R - 17的RNA指导的蛋白质合成。该因子也抑制多聚尿苷酸（poly(U)）指导的翻译，但需要更高的浓度。当在R - 17 RNA指导的翻译开始后添加该因子时，抑制的开始会延迟。多核糖体的不依赖起始的翻译不受抑制。该因子抑制N - 甲酰甲硫氨酰 - tRNAfMet与70S核糖体结合形成70S起始复合物，并从预先形成的复合物中释放N - 甲酰甲硫氨酰 - tRNAfMet。该因子不会阻止N - 甲酰甲硫氨酰嘌呤霉素的形成。得出的结论是，该因子特异性地抑制翻译起始。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab5/348449/b845fcaec5e7/pnas00665-0497-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab5/348449/b845fcaec5e7/pnas00665-0497-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fab5/348449/b845fcaec5e7/pnas00665-0497-a.jpg

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引用本文的文献

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Effects of growth conditions and mutations in RNA polymerase on translational activity in vitro in Escherichia coli.生长条件及RNA聚合酶突变对大肠杆菌体外翻译活性的影响。
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本文引用的文献

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Role of guanosine 5'-triphosphate in the initiation of Peptide synthesis, I. Synthesis of formylmethionyl-puromycin.鸟苷 5'-三磷酸在肽合成起始中的作用，I. 甲酰甲硫氨酰-嘌呤霉素的合成
Proc Natl Acad Sci U S A. 1967 Mar;57(3):759-66. doi: 10.1073/pnas.57.3.759.
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RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.RNA密码子与蛋白质合成。三核苷酸对可溶性核糖核酸（sRNA）与核糖体结合的影响。
Science. 1964 Sep 25;145(3639):1399-407. doi: 10.1126/science.145.3639.1399.
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Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
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Initiation of protein synthesis II. A convenient assay for the ribosome-dependent synthesis of N-formyl-C14-methionylpuromycin.蛋白质合成的起始II。一种用于核糖体依赖性合成N-甲酰基-C14-甲硫氨酰嘌呤霉素的简便测定法。
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In vitro protein synthesis: chain initiation.体外蛋白质合成：链起始
Proc Natl Acad Sci U S A. 1966 Jan;55(1):155-61. doi: 10.1073/pnas.55.1.155.
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Mechanism of action of kasugamycin.春日霉素的作用机制。
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Inhibition by kasugamycin of initiation complex formation on 30S ribosomes.春日霉素对30S核糖体上起始复合物形成的抑制作用。
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J Mol Biol. 1968 Apr 14;33(1):231-49. doi: 10.1016/0022-2836(68)90291-x.
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Biochemistry. 1973 Feb;12(4):609-15. doi: 10.1021/bi00728a007.
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