一种用于检测粪便中人类呼肠孤病毒样病原体的微量滴定板固相放射免疫测定法。
A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools.
作者信息
Kalica A R, Purcell R H, Sereno M M, Wyatt R G, Kim H W, Chanock R M, Kapikian A Z
出版信息
J Immunol. 1977 Apr;118(4):1275-9.
PMID:321688
Abstract
The development of a microtiter solid-phase radioimmunoassay for detection of the human reovirus-like (RVL) agent is described. The test is simple to perform and uses small amounts of reagents; a large number of specimens can rapidly be tested in a single test. Both filtered and unfiltered stool suspensions can be employed. The test was as sensitive as immune electron microscopy, and with an appropriate blocking test, was specific for the human RVL agent.
摘要
本文描述了一种用于检测人呼肠孤病毒样（RVL）病原体的微量滴定板固相放射免疫测定法的开发。该测试操作简单，试剂用量少；可以在一次测试中快速检测大量标本。过滤和未过滤的粪便悬液均可使用。该测试与免疫电子显微镜一样灵敏，并且通过适当的阻断试验，对人RVL病原体具有特异性。
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