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酵母线粒体中21S核糖体RNA前体的加工

Processing of precursors of 21S ribosomal RNA from yeast mitochondria.

作者信息

Merten S, Synenki R M, Locker J, Christianson T, Rabinowitz M

出版信息

Proc Natl Acad Sci U S A. 1980 Mar;77(3):1417-21. doi: 10.1073/pnas.77.3.1417.

DOI:10.1073/pnas.77.3.1417
PMID:6990410
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC348506/
Abstract

The transcription and processing of mitochondrial 21S rRNA in a petite strain of Saccharomyces cerevisiae has been examined by electron microscopic analysis of R-loop hybrids and by hybridization of labeled mitochondrial DNA probes to RNA transferred to diazobenzyloxymethyl paper. We have shown the presence of a large [5.1- to 5.4-kilobase (kb)] transcript that appears to be a precursor of mitochondrial 21S rRNA. This transcript contains sequences homologous to those of the mature 21S rRNA, to the intervening sequence present in the gene, and to additional sequences at the 3' end of the molecule. Our data suggest that this precursor of 21S rRNA is processed in two steps. The intron sequence is usually excised first, followed by removal of the extra 3' sequences. In some cases, however, the 3' extension is first removed and the intron sequence is then excised. Both pathways appear to lead to formation of the 3.1-kb mature 21S rRNA and a stable 1.2-kb intron transcript. Similar results were obtained with grande MH41-7B mitochondrial RNA by RNA transfer hybridization. We have also observed a number of additional transcripts that may be normal processing intermediates or may result from faulty cleavage-ligation during excision of the intervening sequence.

摘要

通过对R环杂交体进行电子显微镜分析，以及将标记的线粒体DNA探针与转移至重氮苄氧基甲基纸上的RNA进行杂交，我们研究了酿酒酵母小菌落菌株中线粒体21S rRNA的转录和加工过程。我们发现存在一种大型[5.1至5.4千碱基（kb）]转录本，它似乎是线粒体21S rRNA的前体。该转录本包含与成熟21S rRNA、基因中存在的间隔序列以及分子3'端其他序列同源的序列。我们的数据表明，这种21S rRNA前体的加工分两步进行。内含子序列通常首先被切除，随后去除额外的3'序列。然而，在某些情况下，首先去除3'延伸部分，然后切除内含子序列。这两条途径似乎都导致形成3.1 kb的成熟21S rRNA和稳定的1.2 kb内含子转录本。通过RNA转移杂交，在大菌落MH41 - 7B线粒体RNA中也获得了类似结果。我们还观察到一些其他转录本，它们可能是正常的加工中间体，也可能是在间隔序列切除过程中切割 - 连接错误导致的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2109/348506/3c6b75e8eb25/pnas00666-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2109/348506/5a4b8572d6c0/pnas00666-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2109/348506/3c6b75e8eb25/pnas00666-0197-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2109/348506/5a4b8572d6c0/pnas00666-0196-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2109/348506/3c6b75e8eb25/pnas00666-0197-a.jpg

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Processing of precursors of 21S ribosomal RNA from yeast mitochondria.酵母线粒体中21S核糖体RNA前体的加工
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Physical mapping of the yeast mitochondrial genome: derivation of the fine structure and gene map of strain D273-10B and comparison with a strain (MH41-7B) differing in genome size.酵母线粒体基因组的物理图谱：D273 - 10B菌株精细结构和基因图谱的推导以及与基因组大小不同的菌株（MH41 - 7B）的比较。
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Fine structure of the 21S ribosomal RNA region on yeast mitochondria DNA. I. Construction of the physical map and localization of the cistron for the 21S mitochondrial ribosomal RNA.酵母线粒体DNA上21S核糖体RNA区域的精细结构。I. 21S线粒体核糖体RNA顺反子的物理图谱构建及定位
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RNA processing and expression of an intron-encoded protein in yeast mitochondria: role of a conserved dodecamer sequence.酵母线粒体中内含子编码蛋白的RNA加工与表达：一个保守十二聚体序列的作用
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Physical mapping of genes on yeast mitochondrial DNA: localization of antibiotic resistance loci, and rRNA and tRNA genes.酵母线粒体DNA上基因的物理图谱：抗生素抗性位点以及rRNA和tRNA基因的定位
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The DEAD box protein Mrh4 functions in the assembly of the mitochondrial large ribosomal subunit.DEAD 框蛋白 Mrh4 在线粒体大亚基组装中发挥作用。

本文引用的文献

1
Biogenesis of Mitochondria: Analysis of Deletion of Mitochondrial Antibiotic Resistance Markers in Petite Mutants of Saccharomyces cerevisiae.线粒体的生物发生：酿酒酵母小菌落突变体中线粒体抗生素抗性标记缺失的分析
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Electron microscopic and renaturation kinetic analysis of mitochondrial DNA of cytoplasmic petite mutants of Saccharomyces cerevisiae.酿酒酵母细胞质小菌落突变体线粒体DNA的电子显微镜及复性动力学分析
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Hybridization of mitochondrial transfer RNA's with mitochondrial and nuclear DNA of grande (wild type) yeast.
Cell Metab. 2013 Nov 5;18(5):712-25. doi: 10.1016/j.cmet.2013.10.007.
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Mitochondrial DNA from Podospora anserina : IV. The large ribosomal RNA gene contains two long intervening sequences.灰盖鬼伞（Podospora anserina）的线粒体 DNA：IV. 大亚基 rRNA 基因含有两个长的间隔序列。
Curr Genet. 1983 Apr;7(2):151-7. doi: 10.1007/BF00365641.
5
The suv3 nuclear gene product is required for the in vivo processing of the yeast mitochondrial 21s rRNA transcripts containing the r1 intron.酵母线粒体中包含r1内含子的21s rRNA转录本的体内加工需要suv3核基因产物。
Curr Genet. 1995 Feb;27(3):234-8. doi: 10.1007/BF00326154.
6
RNA synthesis in isolated yeast mitochondria.分离的酵母线粒体中的RNA合成
Nucleic Acids Res. 1981 Dec 11;9(23):6369-77. doi: 10.1093/nar/9.23.6369.
7
Oligoadenylate is present in the mitochondrial RNA of Saccharomyces cerevisiae.寡腺苷酸存在于酿酒酵母的线粒体RNA中。
Mol Cell Biol. 1982 Apr;2(4):450-6. doi: 10.1128/mcb.2.4.450-456.1982.
8
Synthesis and processing of ribosomal RNA in isolated yeast mitochondria.核糖体RNA在分离出的酵母线粒体中的合成与加工
Nucleic Acids Res. 1981 Dec 11;9(23):6379-90. doi: 10.1093/nar/9.23.6379.
9
Use of a synthetic DNA oligonucleotide to probe the precision of RNA splicing in a yeast mitochondrial petite mutant.使用合成DNA寡核苷酸探测酵母线粒体小菌落突变体中RNA剪接的精确性。
Nucleic Acids Res. 1981 Sep 25;9(18):4475-83. doi: 10.1093/nar/9.18.4475.
10
A mitochondrial locus is necessary for the synthesis of mitochondrial tRNA in the yeast Saccharomyces cerevisiae.线粒体基因座对于酿酒酵母中线粒体tRNA的合成是必需的。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4743-7. doi: 10.1073/pnas.78.8.4743.
线粒体转运RNA与大型（野生型）酵母的线粒体DNA和核DNA的杂交
J Mol Biol. 1972 Feb 14;63(3):431-40. doi: 10.1016/0022-2836(72)90438-x.
4
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
5
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.通过用DNA聚合酶I进行切口平移在体外将脱氧核糖核酸标记至高比活性。
J Mol Biol. 1977 Jun 15;113(1):237-51. doi: 10.1016/0022-2836(77)90052-3.
6
Mapping of mitochondrial genes in Saccharomyces cerevisiae. Populations and pedigree analysis of retention or loss of four genetic markers in Rho-cells.酿酒酵母中线粒体基因的定位。Rho⁻细胞中四个遗传标记保留或丢失的群体及家系分析。
Mol Gen Genet. 1976 Jul 23;146(2):117-32. doi: 10.1007/BF00268080.
7
Hybridization of RNA to double-stranded DNA: formation of R-loops.RNA与双链DNA的杂交：R环的形成。
Proc Natl Acad Sci U S A. 1976 Jul;73(7):2294-8. doi: 10.1073/pnas.73.7.2294.
8
Deletion mapping of mitochondrial transfer RNA genes in Saccharomyces cerevisiae by means of cytoplasmic petite mutants.利用细胞质小菌落突变体对酿酒酵母线粒体转移RNA基因进行缺失定位
Mol Gen Genet. 1976 Apr 23;145(1):7-17. doi: 10.1007/BF00331551.
9
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.通过转移至重氮苄氧基甲基纸并与DNA探针杂交来检测琼脂糖凝胶中特定RNA的方法。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4. doi: 10.1073/pnas.74.12.5350.
10
Assembly of the mitochondrial membrane system: sequences of yeast mitochondrial valine and an unusual threonine tRNA gene.线粒体膜系统的组装：酵母线粒体缬氨酸和一个不寻常的苏氨酸tRNA基因的序列
Cell. 1979 Sep;18(1):47-53. doi: 10.1016/0092-8674(79)90352-0.