Day J F, Ingebretsen C G, Ingebretsen W R, Baynes J W, Thorpe S R
Diabetes. 1980 Jul;29(7):524-7. doi: 10.2337/diab.29.7.524.
The relationship between concentrations of blood glucose and nonenzymatically glucosylated serum proteins was studied in rats with alloxan-induced diabetes of varying severity. Fasting serum glucose correlated strongly with both glucosylated albumin (r = 0.91, P less than 0.001) and glucosylated serum protein (r = 0.93, P less than 0.001). The relative rates of response of serum protein and hemoglobin glycosylation to changes in blood glucose were also compared. Following withdrawal of insulin from diabetic rats, the half-times to reach new steady state levels of blood glucose, glucosylated serum proteins, and glycohemoglobins were about 2, 3, and 8 days, respectively. Similarly, on reinstitution of insulin therapy, the half-times for these same indices to return to baseline values were 2, 3.5, and 15 days, respectively. Changes in glucosylated albumin were more sensitive than glycohemoglobins to changes in serum glucose, consistent with the observation that albumin was glucosylated at about 10 times the rate for hemoglobin in incubations in vitro. These data indicate that glucosylated serum protein measurements can serve as a sensitive, short-term integrator of blood glucose homeostasis in diabetes.
在患有不同严重程度的四氧嘧啶诱导糖尿病的大鼠中，研究了血糖浓度与非酶糖基化血清蛋白之间的关系。空腹血清葡萄糖与糖基化白蛋白（r = 0.91，P < 0.001）和糖基化血清蛋白（r = 0.93，P < 0.001）均密切相关。还比较了血清蛋白和血红蛋白糖基化对血糖变化的相对反应速率。糖尿病大鼠停用胰岛素后，血糖、糖基化血清蛋白和糖化血红蛋白达到新稳态水平的半衰期分别约为2天、3天和8天。同样，重新开始胰岛素治疗后，这些相同指标恢复到基线值的半衰期分别为2天、3.5天和15天。糖基化白蛋白的变化比糖化血红蛋白对血清葡萄糖变化更敏感，这与体外孵育中白蛋白糖基化速率约为血红蛋白10倍的观察结果一致。这些数据表明，糖基化血清蛋白测量可作为糖尿病中血糖稳态的敏感短期综合指标。
