Desaymard C, Shinton R A, Waldmann H
Immunology. 1980 Jan;39(1):61-5.
Incubation of unprimed spleen B cells with high concentrations of hapten-conjugates resulted in the induction of specific unresponsiveness or tolerance to a subsequent encounter with the hapten on a potentially immunogenic carrier. This process of tolerance induction could occur in the absence of extracellular calcium. In contrast B-cell activation to both proliferation and subsequent antibody secretion is known to be calcium dependent. This means that either (1) the decisions which determine immunity and tolerance in B cells are mediated through totally distinct signalling pathways, or that (2) if tolerance and immunity depend on same common signalling events, then the commitment of B cells to switch on or off must be determined at a very early stage.
将未致敏的脾脏B细胞与高浓度的半抗原偶联物一起孵育，会导致对随后在潜在免疫原性载体上遇到的半抗原产生特异性无反应性或耐受性。这种耐受性诱导过程可以在细胞外钙缺失的情况下发生。相比之下，已知B细胞对增殖和随后抗体分泌的激活是钙依赖性的。这意味着要么(1)决定B细胞免疫和耐受的决策是通过完全不同的信号通路介导的，要么(2)如果耐受和免疫依赖于相同的共同信号事件，那么B细胞开启或关闭的决定必须在非常早期阶段就确定。
