Rhodes J C, Howard D H
Infect Immun. 1980 Mar;27(3):910-4. doi: 10.1128/iai.27.3.910-914.1980.
Arginine auxotrophs were isolated in both mating types of Cryptococcus neoformans. In both mutants, the auxotrophy was due to the lack of active argininosuccinate lyase. The virulence in mice of the mutants was compared with that of the wild type. One auxotroph displayed a loss of virulence which appeared to be related to the presence of another mutation, one which prevented normal cell separation after budding. The other auxotroph had reduced virulence compared with the wild type, but a variant isolated from it by mouse passage had virulence equivalent to that of the wild type while maintaining the auxotrophic requirements.
在新型隐球菌的两种交配型中均分离出精氨酸营养缺陷型菌株。在这两种突变体中，营养缺陷是由于缺乏活性精氨琥珀酸裂解酶所致。将这些突变体在小鼠中的毒力与野生型进行了比较。一种营养缺陷型表现出毒力丧失，这似乎与另一个突变的存在有关，该突变阻止了出芽后正常的细胞分离。另一种营养缺陷型与野生型相比毒力降低，但通过小鼠传代从其中分离出的一个变体具有与野生型相当的毒力，同时保留了营养缺陷型的需求。
