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人尿中有机酸的自动代谢谱分析。I. 方法描述。

Automated metabolic profiling of organic acids in human urine. I. Description of methods.

作者信息

Gates S C, Dendramis N, Sweeley C C

出版信息

Clin Chem. 1978 Oct;24(10):1674-9.

PMID:699272
Abstract

We describe a complete procedure for separation and mechanized analysis of organic acids in human urine. The acid fraction of urine is separated by anion-exchange chromatography on diethylaminoethyl-Sephadex. Individual acids are identified and measured by use of a gas-chromatography/mass spectrometer/computer system that can clearly distinguish contributions from at least 150 substances in a single sample. We discuss analytical recoveries, contributions from the sample separation process, stability of stored samples before and after processing, and reproducibility of the extraction procedure.

摘要

我们描述了一种用于分离和机械分析人尿中有机酸的完整程序。尿中的酸性成分通过在二乙氨基乙基-葡聚糖凝胶上进行阴离子交换色谱法进行分离。使用气相色谱/质谱仪/计算机系统对各个酸进行鉴定和测量，该系统能够在单个样品中清晰地区分至少150种物质的贡献。我们讨论了分析回收率、样品分离过程的贡献、处理前后储存样品的稳定性以及提取程序的重现性。

相似文献

1
Automated metabolic profiling of organic acids in human urine. I. Description of methods.人尿中有机酸的自动代谢谱分析。I. 方法描述。
Clin Chem. 1978 Oct;24(10):1674-9.
2
Automated metabolic profiling of organic acids in human urine. II. Analysis of urine samples from "healthy" adults, sick children, and children with neuroblastoma.
Clin Chem. 1978 Oct;24(10):1680-9.
3
[Study of the extraction of organic acids from urine. Preliminary step to their gas chromatographic separation (author's transl)].[尿液中有机酸的提取研究。气相色谱分离的初步步骤（作者译）]
J Chromatogr. 1982 Mar 12;228:67-74.
4
Solid-phase extraction with strong anion-exchange columns for selective isolation and concentration of urinary organic acids.
Clin Chem. 1988 Jun;34(6):1077-83.
5
Adsorption of organic acids from amniotic fluid and urine onto silica gel before analysis by gas chromatography and combined gas chromatography/mass spectrometry.
Clin Chem. 1979 Oct;25(10):1814-20.
6
Isolation, identification and quantitation of urinary organic acids.尿有机酸的分离、鉴定和定量分析。
J Chromatogr. 1979 Mar 1;162(3):249-59. doi: 10.1016/s0378-4347(00)81512-1.
7
Evaluation of continuous solvent extraction of organic acids from biological fluids.
Clin Biochem. 1978 Jun;11(3):126-30. doi: 10.1016/s0009-9120(78)90168-6.
8
Screening of organic acids in urine by chemical ionization mass spectrometry.采用化学电离质谱法筛查尿液中的有机酸。
Biomed Mass Spectrom. 1979 Feb;6(2):47-56. doi: 10.1002/bms.1200060203.
9
Improved procedure for the anion-exchange isolation of urinary organic acids.
J Chromatogr. 1981 Mar 13;222(3):337-44. doi: 10.1016/s0378-4347(00)84133-x.
10
Sample preparation with an automated robotic workstation for organic acid analysis by gas chromatography-mass spectrometry.使用自动机器人工作站进行样品制备，用于气相色谱-质谱联用仪分析有机酸。
J Chromatogr B Biomed Appl. 1996 Oct 11;685(1):1-7. doi: 10.1016/0378-4347(96)00136-3.

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