Maret S M, Baseman J B, Folds J D
Clin Exp Immunol. 1980 Jan;39(1):38-43.
Peripheral blood lymphocytes from Treponema pallidum infected rabbits respond poorly to mitogen and specific antigens when cultured in the presence of autologous serum. Reactivity of lymphocytes from the spleen and popliteal lymph nodes of T. pallidum infected rabbits have therefore been examined by lymphocyte transformation using the mitogens phytohaemagglutinin (PHA) and concanavalin A (Con A) and extracts of T. pallidum. Spleen cell populations, both T cell enriched (by nylon wool elution) and non-nylon wool treated, which respond to T. pallidum as early as ten days post infection in normal serum, were suppressed in responses to T. pallidum when cultured in autologous serum. The same lymphocytes responded normally to PHA and Con A. Lymph node cells from infected rabbits responded normally to both T. pallidum antigen and mitogens in either autologous or normal rabbit serum. These data indicate that splenic lymphocytes are sensitive to regulatory factors in autologous serum during the early stages of T. pallidum infection whereas lymph node cells are not.
在自体血清存在的情况下培养时，来自感染梅毒螺旋体兔子的外周血淋巴细胞对有丝分裂原和特异性抗原反应较差。因此，通过使用有丝分裂原植物血凝素（PHA）和刀豆球蛋白A（Con A）以及梅毒螺旋体提取物进行淋巴细胞转化，检测了感染梅毒螺旋体兔子的脾脏和腘窝淋巴结淋巴细胞的反应性。在正常血清中早在感染后十天就对梅毒螺旋体有反应的脾脏细胞群体，无论是富集T细胞的（通过尼龙毛洗脱）还是未经过尼龙毛处理的，当在自体血清中培养时，对梅毒螺旋体的反应都受到抑制。相同的淋巴细胞对PHA和Con A反应正常。感染兔子的淋巴结细胞在自体或正常兔血清中对梅毒螺旋体抗原和有丝分裂原都反应正常。这些数据表明，在梅毒螺旋体感染的早期阶段，脾脏淋巴细胞对自体血清中的调节因子敏感，而淋巴结细胞则不敏感。
