Pavia C S, Folds J D, Baseman J B
Infect Immun. 1977 Dec;18(3):603-11. doi: 10.1128/iai.18.3.603-611.1977.
The blastogenic response of nylon wool-separated peripheral-blood lymphocytes from Treponema pallidum-infected rabbits was tested in vitro with mitogens and T. pallidum antigens. The mitogenic response of the enriched T-cell population to concanavalin A and phytohemagglutinin was depressed during the first 3 to 4 weeks of infection, similar to the pattern observed with unfractionated cells. Shortly thereafter, levels of blastogenesis returned to values of uninfected cultures. Enhanced blast transformation was seen immediately when purified T-cells from infected rabbits were exposed in vitro to T. pallidum antigens. Although these relatively high levels of blastogenesis were maintained for the duration of the experiment, cultures of unfractionated lymphocytes from infected rabbits did not exhibit an increased blastogenic response to the same antigen preparation until 3 to 4 weeks after infection. Autologous serum from infected rabbits decreased the lymphocyte response to T. pallidum antigen. The stimulatory effects of anti-immunoglobulin G and lipopolysaccharide on nylon wool-fractionated or unfractionated lymphocytes from both infected and control rabbits were similar throughout the course of infection. During the first 6 weeks of experimental disease, there was a 25 to 31% increase in the number of lymphocytes circulating in the peripheral blood of T. pallidum-infected rabbits.
用丝裂原和梅毒螺旋体抗原在体外检测梅毒螺旋体感染兔经尼龙毛分离的外周血淋巴细胞的增殖反应。在感染的最初3至4周内，富集的T细胞群体对刀豆球蛋白A和植物血凝素的促有丝分裂反应受到抑制，这与未分离细胞所观察到的模式相似。此后不久，增殖水平恢复到未感染培养物的值。当将感染兔的纯化T细胞在体外暴露于梅毒螺旋体抗原时，立即观察到增强的增殖转化。尽管在实验期间这些相对较高的增殖水平得以维持，但感染兔的未分离淋巴细胞培养物直到感染后3至4周才表现出对相同抗原制剂的增殖反应增加。感染兔的自体血清降低了淋巴细胞对梅毒螺旋体抗原的反应。在整个感染过程中，抗免疫球蛋白G和脂多糖对感染兔和对照兔的尼龙毛分离或未分离淋巴细胞的刺激作用相似。在实验性疾病的前6周内，梅毒螺旋体感染兔外周血中循环的淋巴细胞数量增加了25%至31%。
