Knaup G, Pfleiderer G, Bayreuther K
Clin Chim Acta. 1978 Sep 1;88(2):375-83. doi: 10.1016/0009-8981(78)90444-8.
The testing of the type of membrane associated alkaline phosphatase (EC 3.1.3.1) by immunotitration has revealed that there is a shift from a liver-like type of alkaline phosphatase in normal cell cultures of the human diploid fibroblast cell strains WI 26 and WI 38 to a placenta-like variant in cultures of the same cell strains after the transformation by the DNA-tumor virus SV 40, the WI 26 SV 40 and the WI 38 SV 40 cell lines. The immunologically detectable switch-over has been confirmed by measuring the apparent Michaelis constant and the heat stability of the AP from normal and transformed cells. Liver-like AP is heat labile and has an apparent Michaelis constant for p-nitrophenylphosphate (about 4.0 X 10(-4) M). The placenta-like AP shows heat stability and a lower apparent KM (about 2.2 X 10(-4) M). The appearance of the so-called Regan enzyme of AP in some human tumors in vivo is discussed in this connection.
通过免疫滴定法对膜相关碱性磷酸酶（EC 3.1.3.1）类型的检测显示，在人二倍体成纤维细胞株WI 26和WI 38的正常细胞培养物中，碱性磷酸酶呈现肝样类型，而在经DNA肿瘤病毒SV 40转化后的相同细胞株培养物（WI 26 SV 40和WI 38 SV 40细胞系）中，则转变为胎盘样变体。通过测量正常细胞和转化细胞中碱性磷酸酶的表观米氏常数和热稳定性，证实了这种免疫可检测的转变。肝样碱性磷酸酶对热不稳定，对磷酸对硝基苯酯的表观米氏常数约为4.0×10⁻⁴ M。胎盘样碱性磷酸酶具有热稳定性，且表观米氏常数较低（约2.2×10⁻⁴ M）。本文还讨论了体内一些人类肿瘤中所谓碱性磷酸酶的Regan酶的出现情况。
