Ruiz-Vázquez R, Cerdá-Olmedo E
Mol Gen Genet. 1980;178(3):625-31. doi: 10.1007/BF00337870.
A newly-isolated Escherichia coli mutant suffers only about 10% as many mutations as normal strains on exposure to nitrosoguanidine. The responsible mutation, inm-1, maps at approximately minute 79 in the current E. coli genetic map. The mutant is normal for overall growth, nitrosoguanidine lethality, spontaneous mutagenesis, ultraviolet light lethality and mutagenesis, ethyl methanesulfonate lethality and mutagenesis, and the adaptive repair induced by alkylating agents. The existence of this mutation proves that nitrosoguanidine mutagenesis is not merely the result of reactions between the chemical and DNA, but requires specific cellular function(s), and underscores the peculiarity of nitrosoguanidine as a mutagen.
新分离出的大肠杆菌突变体在接触亚硝基胍时发生的突变数量仅为正常菌株的约10%。相关突变基因inm - 1在当前大肠杆菌遗传图谱上大约位于79分钟处。该突变体在总体生长、亚硝基胍致死性、自发诱变、紫外线致死性和诱变、甲基磺酸乙酯致死性和诱变以及烷基化剂诱导的适应性修复方面均表现正常。这一突变的存在证明，亚硝基胍诱变不仅仅是该化学物质与DNA之间反应的结果，还需要特定的细胞功能，这突出了亚硝基胍作为诱变剂的独特性。
