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子囊菌嗜热栖热放线菌类质粒DNA的特性分析与克隆

Characterization and cloning of plasmid like DNA of the ascomycete Podospora anserina.

作者信息

Stahl U, Kück U, Tudzynski P, Esser K

出版信息

Mol Gen Genet. 1980;178(3):639-46. doi: 10.1007/BF00337872.

DOI:10.1007/BF00337872
PMID:6993860
Abstract

The previously reported existence of plasmid-like (pl) DNA in senescent mycelia of Podospora anserina was confirmed using new methodology. Detailed anaysis of bulk DNA has further shown a possible relationship between pl DNA and mt DNA. According to biophysical and electron microscopic experiments the pl DNA was found to consist of oligomeres having a basic unit with a contour length of 0.75 micrometer corresponding to 2.4 kb. To overcome the handicap that pl DNA is only produced in rather small amounts in the aging mycelia, this DNA was cloned in E. coli after insertion into a bacterial plasmid vector, pBR 322. It was possible to isolate a stable hybrid plasmid consisting of the vector and only one integrated monomere of pl DNA. The composition of this hybrid plasmid was confirmed by restriction endonuclease analysis and heteroduplex formation. A restriction map of the pl DNA is presented and its insertion site onto pBR 322 indicated.

摘要

利用新方法证实了先前报道的在栗疫霉衰老菌丝体中存在类质粒（pl）DNA。对大量DNA的详细分析进一步表明了pl DNA与线粒体DNA（mt DNA）之间可能存在的关系。根据生物物理和电子显微镜实验，发现pl DNA由寡聚体组成，其基本单位的轮廓长度为0.75微米，相当于2.4千碱基对。为克服pl DNA仅在衰老菌丝体中少量产生这一障碍，将该DNA插入细菌质粒载体pBR 322后在大肠杆菌中进行克隆。有可能分离出一种由载体和仅一个整合的pl DNA单体组成的稳定杂交质粒。通过限制性内切酶分析和异源双链体形成证实了该杂交质粒的组成。给出了pl DNA的限制性图谱，并标明了其在pBR 322上的插入位点。

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引用本文的文献

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Plasmid-like DNA is part of mitochondrial DNA in Podospora anserina.质体样 DNA 是 Podospora anserina 线粒体 DNA 的一部分。
Curr Genet. 1981 May;3(2):151-6. doi: 10.1007/BF00365719.
2
Mitochondrial DNA amplification in senescent cultures of Podospora anserina: Variability between the retained, amplified sequences.毕赤酵母衰老培养物中线粒体 DNA 的扩增：保留、扩增序列之间的变异性。
Curr Genet. 1981 Apr;3(1):13-21. doi: 10.1007/BF00419575.
3
Transformation to senescence with plasmid like DNA in the ascomycete Podospora anserina.

本文引用的文献

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[Gamma satellite DNA and small twisted circular molecules in yeast Saccharomyces cerevisiae].[酿酒酵母中的γ卫星DNA和小的扭曲环状分子]
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Replication of a bacterial episome under relaxed control.在松弛控制下细菌附加体的复制。
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9
Anatomy of amplified mitochondrial DNA in "Ragged" mutants of Aspergillus amstelodami: Excision points within protein genes and a common 215 bp segment containing a possible origin of replication.aspergillus amstelodami“锯齿状”突变体中扩增线粒体 DNA 的结构：蛋白基因内的切除位点和一个包含可能复制起点的 215bp 共同片段。
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10
The mitochondrial plasmid of Podospora anserina: A mobile intron of a mitochondrial gene.粗糙脉孢菌的线粒体质粒：线粒体基因的移动内含子。
Curr Genet. 1984 May;8(4):299-305. doi: 10.1007/BF00419728.
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J Mol Biol. 1974 Jul 15;86(4):825-41. doi: 10.1016/0022-2836(74)90356-8.
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