Honer W G, Ashwood-Smith M J, Warby C
Mutat Res. 1980 Jun;78(2):137-44. doi: 10.1016/0165-1218(80)90092-0.
Swimming pool water, being chlorinated and exposed to trace organics from use was investigated as a possible source of mutagens using the Salmonella/mammalian-microsome test. Procedures previously described for the extraction of trace organics from water using XAD-2 macroreticular resin were modified to allow quantitative extraction of mutagens. These procedures were superior to freeze-drying and solvent-extraction. Using a base-pair histidine mutant, strain TA100, of Salmonella typhimurium significantly mutagenic responses were observed using concentrates from 3 variations of the extraction procedure. Acidified pool-water extracts eluted with ether or acetone were mutagenic, the former enhanced in the presence of the induced microsomal fraction from rat livers. Non-acidified pool-water extracts eluted with acetone were mutagenic without microsomal activation. These results indicate the presence of more than one mutagen in what is likely a complex mixture of organic molecules in swimming-pool water.
采用沙门氏菌/哺乳动物微粒体试验，对经过氯化处理并接触使用过程中产生的痕量有机物的游泳池水作为诱变剂的可能来源进行了研究。之前描述的使用XAD - 2大孔网状树脂从水中提取痕量有机物的程序进行了修改，以实现诱变剂的定量提取。这些程序优于冷冻干燥和溶剂萃取。使用鼠伤寒沙门氏菌的碱基对组氨酸突变株TA100，从3种不同提取程序的浓缩物中观察到了显著的诱变反应。用乙醚或丙酮洗脱的酸化泳池水提取物具有诱变性，前者在存在大鼠肝脏诱导微粒体组分的情况下增强。用丙酮洗脱的未酸化泳池水提取物在无微粒体激活的情况下具有诱变性。这些结果表明，在游泳池水这种可能是有机分子的复杂混合物中存在不止一种诱变剂。
