Bartlett G L, Kreider J W, Purnell D M, Katsilas D C
Cancer. 1980 Aug 1;46(3):488-96. doi: 10.1002/1097-0142(19800801)46:3<488::aid-cncr2820460312>3.0.co;2-m.
The line 10 hepatoma of strain 2 guinea pigs was used in order to evaluate the adjuvant efficacy of eight Bacillus Calmette-Guérin (BCG) suspensions which differed in strain, method of preservation, or dosage. Lyophilized Tice BCG was consistently the most effective preparation. Each strain had adjuvant activity in at least one experiment. The method of preservation of BCG (fresh-frozen vs. lyophilized) did not have a consistent, a predictable influence on adjuvanticity. A ten-fold increase in the dosage of BCG or of the whole vaccine was not supraoptimal.
为了评估八种卡介苗（BCG）悬液的佐剂效果，使用了2系豚鼠的10号肝癌模型。这八种卡介苗悬液在菌株、保存方法或剂量上存在差异。冻干的Tice卡介苗始终是最有效的制剂。每种菌株在至少一项实验中都具有佐剂活性。卡介苗的保存方法（新鲜冷冻与冻干）对佐剂活性没有一致的、可预测的影响。卡介苗或整个疫苗剂量增加十倍并非超最佳剂量。
