Hyde B, Burgett M W, Maggio E T
Clin Chim Acta. 1980 May 9;103(3):393-8. doi: 10.1016/0009-8981(80)90159-x.
A solid-phase fluoroimmunoassay (FIAX¿) for the detection and quantitation of human antibodies to Toxoplasma gondii is described. The method is a modification of the procedure of Walls and Barnhart [1] which differs from the earlier test in that it uses a dual rather than single surface StiQTM Sampler, having both an antigen and a control surface. Each sampler is carried through four steps: reaction with diluted serum, a buffer wash, reaction with FITC-labeled goat anti-human IgG, and a final buffer wash. The fluorescence of each surface is measured using a dedicated surface-reading fluorometer. Serum titers are interpolated from a standard curve. The antibody titers of 233 sera were determined by both the indirect immunofluorescent antibody (IFA) and the FIAX¿ assays. The titers determined by the two tests agreed within one four-fold dilution for 226 (97%) of the sera. For replicates (n = 21) of the sera with low, mid and high antibody titers the coefficients of variation were 13.6, 10.0, and 12.3% respectively.
本文描述了一种用于检测和定量人抗弓形虫抗体的固相荧光免疫测定法（FIAX™）。该方法是对Walls和Barnhart [1]方法的改进，与早期测试的不同之处在于它使用了具有抗原和对照表面的双表面而非单表面StiQ™采样器。每个采样器经过四个步骤：与稀释血清反应、缓冲液洗涤、与异硫氰酸荧光素（FITC）标记的山羊抗人IgG反应以及最后的缓冲液洗涤。使用专用的表面读数荧光计测量每个表面的荧光。血清滴度通过标准曲线进行插值。通过间接免疫荧光抗体（IFA）和FIAX™测定法测定了233份血清的抗体滴度。两种测试所测定的滴度在226份（97%）血清的一个四倍稀释范围内一致。对于低、中、高抗体滴度血清的重复样本（n = 21），变异系数分别为13.6%、10.0%和12.3%。
