用于检测弓形虫IgG抗体的FIAX半自动荧光免疫分析系统的评估

Evaluation of the FIAX semiautomatic fluorescent immunoassay system for the detection of IgG antibodies to Toxoplasma gondii.

作者信息

Carlson L G, Plorde J J

出版信息

Diagn Microbiol Infect Dis. 1983 Sep;1(3):233-9. doi: 10.1016/0732-8893(83)90023-8.

Abstract

A commercially available solid phase fluoroimmunoassay system (FIAX, International Diagnostic Technology, Santa Clara, CA) was evaluated in parallel with a standard indirect fluorescent-antibody test (IFAT) for the detection of IgG antibodies to Toxoplasma gondii. Titers obtained by the FIAX method on the 101 patient samples correlated well with the standard procedure. Sensitivity of the FIAX system was 96% and specificity was 93%. A method for measuring serum titer reproducibility applicable to both conventional doubling dilution and to automated procedures was employed, allowing a meaningful comparison of precision in the two systems. It was found that titer reproducibility in the FIAX system was better than that of the IFAT and exceeded that expected with most conventional double-dilution serological procedures. The FIAX system will most benefit those laboratories with relatively large numbers of samples which can be batch tested.

摘要

对一种市售的固相荧光免疫分析系统（FIAX，国际诊断技术公司，加利福尼亚州圣克拉拉）与标准间接荧光抗体试验（IFAT）进行了平行评估，以检测弓形虫IgG抗体。通过FIAX方法在101份患者样本上获得的滴度与标准程序相关性良好。FIAX系统的敏感性为96%，特异性为93%。采用了一种适用于传统倍比稀释和自动化程序的测量血清滴度重复性的方法，从而能够对两种系统的精密度进行有意义的比较。结果发现，FIAX系统的滴度重复性优于IFAT，并且超过了大多数传统倍比稀释血清学程序的预期。FIAX系统将使那些有相对大量样本且可进行批量检测的实验室受益最大。