酶联免疫测定法和定量间接荧光抗体试验与传统间接荧光抗体试验检测弓形虫抗体的比较。
Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii.
作者信息
Violand S A, Mitchell T G, Kleeman K T
出版信息
J Clin Microbiol. 1982 Aug;16(2):341-4. doi: 10.1128/jcm.16.2.341-344.1982.
Abstract
Two new methods for the detection of antibodies to Toxoplasma gondii, an enzyme-linked immunosorbent assay and a quantitative immunofluorescence assay, were evaluated and compared with the conventional indirect fluorescent-antibody slide test. Each of 100 human sera was assayed twice by the three procedures. Both the enzyme-linked immunosorbent assay and the quantitative immunofluorescence assay correlated well with serologically positive (indirect fluorescent-antibody titer greater than or equal to 1:32) and negative sera. The enzyme-linked immunosorbent assay was more specific, but less sensitive, than the quantitative immunofluorescence assay. However, the quantitative immunofluorescence assay was more reproducible and more rapid than the enzyme-linked immunosorbent assay.
摘要
评估了两种检测弓形虫抗体的新方法，即酶联免疫吸附测定法和定量免疫荧光测定法，并与传统的间接荧光抗体玻片试验进行了比较。用这三种方法对100份人血清样本各进行了两次检测。酶联免疫吸附测定法和定量免疫荧光测定法与血清学阳性（间接荧光抗体效价大于或等于1:32）和阴性血清的相关性都很好。酶联免疫吸附测定法比定量免疫荧光测定法更具特异性，但敏感性较低。然而，定量免疫荧光测定法比酶联免疫吸附测定法具有更高的重复性且检测速度更快。
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