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弓形虫病的自动免疫荧光检测

Automated immunofluorescence test for toxoplasmosis.

作者信息

Gordon M A, Duncan R A, Kingsley L C

出版信息

J Clin Microbiol. 1981 Feb;13(2):283-5. doi: 10.1128/jcm.13.2.283-285.1981.

DOI:10.1128/jcm.13.2.283-285.1981
PMID:7009636
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC273778/
Abstract

A soluble-antigen fluorescent-antibody technique for serodiagnosis of toxoplasmosis, employing a special fluorometer (FIAX; International Diagnostic Technology, Santa Clara, Calif.), was evaluated in comparison with a standard indirect fluorescent-antibody method which uses a fluorescence microscope. Titers obtained by the new method on 324 serum samples correlated very well with those of the conventional procedure. There was good intralaboratory and interlaboratory agreement in runs on selected samples. The FIAX method is simple, rapid, and reliable and has the advantage of objectivity.

摘要

一种用于弓形虫病血清诊断的可溶性抗原荧光抗体技术,采用一种特殊的荧光计(FIAX;国际诊断技术公司,加利福尼亚州圣克拉拉),与使用荧光显微镜的标准间接荧光抗体方法相比进行了评估。通过新方法对324份血清样本测得的滴度与传统方法测得的滴度相关性非常好。在对选定样本的检测中,实验室内部和实验室之间的结果一致性良好。FIAX方法简单、快速且可靠,具有客观性的优点。

相似文献

1
Automated immunofluorescence test for toxoplasmosis.弓形虫病的自动免疫荧光检测
J Clin Microbiol. 1981 Feb;13(2):283-5. doi: 10.1128/jcm.13.2.283-285.1981.
2
Titration of human serum antibodies to Toxoplasma gondii with a simple fluorometric assay.用一种简单的荧光测定法滴定人血清中抗弓形虫抗体。
J Clin Microbiol. 1978 Feb;7(2):234-5. doi: 10.1128/jcm.7.2.234-235.1978.
3
Evaluation of the FIAX semiautomatic fluorescent immunoassay system for the detection of IgG antibodies to Toxoplasma gondii.用于检测弓形虫IgG抗体的FIAX半自动荧光免疫分析系统的评估
Diagn Microbiol Infect Dis. 1983 Sep;1(3):233-9. doi: 10.1016/0732-8893(83)90023-8.
4
[Demonstration of toxoplasma antibody with the aid of immunofluorescence].[借助免疫荧光法检测弓形虫抗体]
Zentralbl Bakteriol Orig A. 1974;227(1-4):294-7.
5
A solid-phase fluoroimmunoassay for the quantitation of antibodies to Toxoplasma gondii.一种用于定量检测抗弓形虫抗体的固相荧光免疫测定法。
Clin Chim Acta. 1980 May 9;103(3):393-8. doi: 10.1016/0009-8981(80)90159-x.
6
[Reproducibility of of indirect immunofluorescence reaction in the study of serum antibodies to Toxoplasma gondii, using 4 different strains of the parasite as antigen].[以4种不同弓形虫株作为抗原,研究弓形虫血清抗体时间接免疫荧光反应的可重复性]
Mem Inst Oswaldo Cruz. 1980;75(1-2):89-97. doi: 10.1590/s0074-02761980000100009.
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[Indirect fluorescent antibody reaction in the determination of human Toxoplasma antibodies].[间接荧光抗体反应在人弓形虫抗体测定中的应用]
Z Gesamte Hyg. 1971 Oct;17(10):750-4.
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[Serodiagnosis of toxoplasmosis by indirect immunofluorescence, complement fixation and its relationship with IgM anti-toxoplasma antibodies].[通过间接免疫荧光法和补体结合法进行弓形虫病的血清学诊断及其与抗弓形虫IgM抗体的关系]
Bull Soc Pathol Exot Filiales. 1974 Mar-Apr;67(2):175-83.
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[Comparative studies of the Sabin-Feldman and immunofluorescence tests for toxoplasma antibodies (author's transl)].弓形虫抗体的萨宾-费尔德曼试验与免疫荧光试验的比较研究(作者译)
Dtsch Med Wochenschr. 1974 May;99(18):949-52. doi: 10.1055/s-0028-1107870.
10
[Conjugation of toxoplasma antibody-containing sera with fluorescein isothiocyanate. Complement fixation test, indirect fluorescent antibody reaction and serum dye test as test criteria for the sera to be selected].
Zentralbl Bakteriol Orig. 1970;213(2):263-9.

引用本文的文献

1
Evaluation of a solid-phase immunofluorescence assay for detection of antibodies to Legionella pneumophila.用于检测嗜肺军团菌抗体的固相免疫荧光测定法的评估
J Clin Microbiol. 1982 Jul;16(1):202-4. doi: 10.1128/jcm.16.1.202-204.1982.
2
Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxoplasma gondii.酶联免疫测定法和定量间接荧光抗体试验与传统间接荧光抗体试验检测弓形虫抗体的比较。
J Clin Microbiol. 1982 Aug;16(2):341-4. doi: 10.1128/jcm.16.2.341-344.1982.
3
Evaluation of commercial serodiagnostic kits for toxoplasmosis.弓形虫病商用血清学诊断试剂盒的评估
J Clin Microbiol. 1987 Dec;25(12):2262-5. doi: 10.1128/jcm.25.12.2262-2265.1987.

本文引用的文献

1
Dyes as Microchemical Indicators of a New Immunity Phenomenon Affecting a Protozoon Parasite (Toxoplasma).作为一种影响原生动物寄生虫(弓形虫)的新免疫现象的微化学指示剂的染料。
Science. 1948 Dec 10;108(2815):660-3. doi: 10.1126/science.108.2815.660.
2
SOLUBLE ANTIGEN FLUORESCENT-ANTIBODY TECHNIQUE.可溶性抗原荧光抗体技术
Appl Microbiol. 1965 Jul;13(4):552-8. doi: 10.1128/am.13.4.552-558.1965.
3
Indirect fluorescent antibody tests for parasitic diseases. IV. Statistical study of variation in the indirect fluorescent antibody (IFA) test for toxoplasmosis.寄生虫病的间接荧光抗体试验。IV. 弓形虫病间接荧光抗体(IFA)试验变异的统计学研究。
Am J Epidemiol. 1967 Sep;86(2):401-7. doi: 10.1093/oxfordjournals.aje.a120750.
4
Titration of human serum antibodies to Toxoplasma gondii with a simple fluorometric assay.用一种简单的荧光测定法滴定人血清中抗弓形虫抗体。
J Clin Microbiol. 1978 Feb;7(2):234-5. doi: 10.1128/jcm.7.2.234-235.1978.