用活的鼠麻风杆菌和超声处理的杆菌免疫后，C57BL小鼠的保护性免疫和迟发型超敏反应。
Protective immunity and delayed-type hypersensitivity in C57BL mice after immunization with live Mycobacterium lepraemurium and sonicated bacilli.
作者信息
Closs O, Løvik M
出版信息
Infect Immun. 1980 Jul;29(1):17-23. doi: 10.1128/iai.29.1.17-23.1980.
The immunizing effects of live Mycobacterium lepraemurium (MLM) and bacillary sonic extract (MLMSon) were compared in C57BL mice. MLMSon-immunized mice developed a delayed-type hypersensitivity (DTH) reaction when tested in the footpad with diluted MLMSon. The ability to develop a DTH reaction was transferable with immune cells but not with serum. Footpad testing with live MLM and MLMSon indicated that the specificity of the DTH response induced by MLMSon was different from that induced by infection with live bacilli. Two weeks after footpad inoculation with live MLM, MLMSon-immunized mice developed a strong local reaction to the bacilli. No local reaction developed in these mice after injection of the same number of heat-killed MLM. Studies of bacillary growth after inoculation with live MLM indicated that the bacilli did not multiply in micr previously inoculated with live MLM, but that they multiplied for about 2 weeks in LMLSon-immunized mice versus 4 weeks in the controls. The results suggest that immunization with MLMSon does not by itself induce a protective immune response, but creates a state in which the development of protective immunity is accelerated.
在C57BL小鼠中比较了活的鼠麻风杆菌（MLM）和杆菌超声提取物（MLMSon）的免疫效果。用稀释的MLMSon对足垫进行检测时，经MLMSon免疫的小鼠出现了迟发型超敏反应（DTH）。产生DTH反应的能力可通过免疫细胞传递，但不能通过血清传递。用活的MLM和MLMSon进行足垫检测表明，MLMSon诱导的DTH反应的特异性与活杆菌感染诱导的不同。在用活的MLM进行足垫接种两周后，经MLMSon免疫的小鼠对杆菌产生了强烈的局部反应。在注射相同数量的热灭活MLM后，这些小鼠未出现局部反应。对接种活的MLM后的杆菌生长研究表明，杆菌在先前接种过活的MLM的小鼠中不繁殖，但在经MLMSon免疫的小鼠中繁殖约2周，而在对照小鼠中繁殖4周。结果表明，用MLMSon免疫本身不会诱导保护性免疫反应，但会创造一种加速保护性免疫发展的状态。
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