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高温下肺炎克雷伯菌中固氮作用的抑制

Repression of nitrogen fixation in Klebsiella pneumoniae at high temperature.

作者信息

Brooks S J, Collins J J, Brill W J

出版信息

J Bacteriol. 1984 Feb;157(2):460-4. doi: 10.1128/jb.157.2.460-464.1984.

DOI:10.1128/jb.157.2.460-464.1984
PMID:6363385
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC215270/
Abstract

The effect of elevated growth temperature on nif mRNA synthesis, nif protein synthesis, and nitrogenase activity was investigated in wild-type and NifL- strains of Klebsiella pneumoniae. Nitrogenase activity is not affected at 41 degrees C; however, nitrogenase is not synthesized at that temperature. Transcription of three nif operons studied is repressed at 41 degrees C. We show that the nifL protein is not required for repression by high temperature and propose that repression of nif at 41 degrees C results from a reversible inactivation of the nifA gene product.

摘要

在肺炎克雷伯菌的野生型和NifL -菌株中，研究了生长温度升高对固氮酶mRNA合成、固氮酶蛋白合成以及固氮酶活性的影响。在41摄氏度时，固氮酶活性不受影响；然而，在该温度下不合成固氮酶。所研究的三个固氮操纵子的转录在41摄氏度时受到抑制。我们表明高温抑制作用不需要NifL蛋白，并提出在41摄氏度时固氮酶基因的抑制是由于固氮酶A基因产物的可逆失活所致。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d12c/215270/69fadeba1499/jbacter00237-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d12c/215270/69fadeba1499/jbacter00237-0126-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d12c/215270/69fadeba1499/jbacter00237-0126-a.jpg

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本文引用的文献

1
Nitrogen control of the nif regulon in Klebsiella pneumoniae: involvement of the ntrA gene and analogies between ntrC and nifA.肺炎克雷伯菌中固氮基因簇的氮调控：ntrA基因的作用以及ntrC与nifA之间的相似性
EMBO J. 1983;2(1):39-44. doi: 10.1002/j.1460-2075.1983.tb01377.x.
2
Positive involvement of ppGpp in derepression of the nif operon in Klebsiella pneumoniae.ppGpp在肺炎克雷伯菌中对固氮酶操纵子去阻遏的正向作用。
Mol Gen Genet. 1982;185(2):198-204. doi: 10.1007/BF00330786.
3
A new model for nitrogen control.一种新的氮控制模型。
不同因素对工程化大肠杆菌78-7固氮酶活性的影响。
World J Microbiol Biotechnol. 2015 Jun;31(6):921-7. doi: 10.1007/s11274-015-1846-x. Epub 2015 Apr 8.
4
Using synthetic biology to distinguish and overcome regulatory and functional barriers related to nitrogen fixation.利用合成生物学区分和克服与固氮相关的调控和功能障碍。
PLoS One. 2013 Jul 25;8(7):e68677. doi: 10.1371/journal.pone.0068677. Print 2013.
5
Effects of temperature stress on bean-nodulating Rhizobium strains.温度胁迫对结瘤根瘤菌菌株的影响。
Appl Environ Microbiol. 1994 Apr;60(4):1206-12. doi: 10.1128/aem.60.4.1206-1212.1994.
6
Importance of cis determinants and nitrogenase activity in regulated stability of the Klebsiella pneumoniae nitrogenase structural gene mRNA.顺式作用元件和固氮酶活性在肺炎克雷伯菌固氮酶结构基因mRNA调控稳定性中的重要性。
J Bacteriol. 1999 Jun;181(12):3751-60. doi: 10.1128/JB.181.12.3751-3760.1999.
7
Regulation of nif gene expression in Enterobacter agglomerans: nucleotide sequence of the nifLA operon and influence of temperature and ammonium on its transcription.成团肠杆菌中固氮基因表达的调控：nifLA操纵子的核苷酸序列以及温度和铵对其转录的影响。
Mol Gen Genet. 1995 Dec 20;249(6):629-36. doi: 10.1007/BF00418032.
8
Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes.纯化的NIFA（一种固氮基因的转录激活因子）的活性。
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2266-70. doi: 10.1073/pnas.90.6.2266.
9
Temperature sensitivity of a nifA-like gene in Enterobacter cloacae.阴沟肠杆菌中一个类nifA基因的温度敏感性
J Bacteriol. 1986 Apr;166(1):357-9. doi: 10.1128/jb.166.1.357-359.1986.
10
Control of Klebsiella pneumoniae nif mRNA synthesis.肺炎克雷伯菌固氮酶mRNA合成的调控
J Bacteriol. 1985 Jun;162(3):1186-90. doi: 10.1128/jb.162.3.1186-1190.1985.
Nature. 1982 Jun 3;297(5865):362-3. doi: 10.1038/297362a0.
4
Nitrogen fixation gene (nifL) involved in oxygen regulation of nitrogenase synthesis in K. pneumoniae.肺炎克雷伯菌中参与固氮酶合成氧调节的固氮基因（nifL）。
Nature. 1981 Apr 2;290(5805):424-6. doi: 10.1038/290424a0.
5
Temperature sensitivity of the regulation of nitrogenase synthesis by Klebsiella pneumoniae.肺炎克雷伯菌对固氮酶合成调控的温度敏感性
J Bacteriol. 1981 Feb;145(2):1116-8. doi: 10.1128/jb.145.2.1116-1118.1981.
6
Regulation of nitrogen fixation in Klebsiella pneumoniae: isolation and characterization of strains with nif-lac fusions.肺炎克雷伯菌中固氮作用的调控：携带nif - lac融合基因菌株的分离与特性分析
J Bacteriol. 1981 Jan;145(1):348-57. doi: 10.1128/jb.145.1.348-357.1981.
7
Mutations in nif genes that cause Klebsiella pneumoniae to be derepressed for nitrogenase synthesis in the presence of ammonium.在铵存在的情况下，导致肺炎克雷伯菌对固氮酶合成去阻遏的固氮基因中的突变。
J Bacteriol. 1980 Nov;144(2):744-51. doi: 10.1128/jb.144.2.744-751.1980.
8
Gene-product relationships of the nif regulon of Klebsiella pneumoniae.
J Bacteriol. 1980 Oct;144(1):210-6. doi: 10.1128/jb.144.1.210-216.1980.
9
Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions.利用基因融合技术对肺炎克雷伯菌固氮（nif）基因簇调控的分析。
Nature. 1980 Jul 10;286(5769):128-32. doi: 10.1038/286128a0.
10
Regulation of nitrogen metabolism genes by nifA gene product in Klebsiella pneumoniae.肺炎克雷伯菌中nifA基因产物对氮代谢基因的调控
Nature. 1983 Jan 27;301(5898):307-13. doi: 10.1038/301307a0.