Hensgens L A, Nieuwenhuis B J, van der Meer R, Meijer A J
Eur J Biochem. 1980;108(1):39-45. doi: 10.1111/j.1432-1033.1980.tb04693.x.
A quantitative study of the contribution of the malate-aspartate and glycerol-3-phosphate cycles to the translocation of reducing equivalents from cytosol to mitochondria during ethanol oxidation has been made in hepatocytes from euthyroid and hyperthyroid rats. 1. In hepatocytes from euthyroid rats both cycles have an almost equal capacity and their relative contribution to total hydrogen transport to the mitochondria depends on the conditions chosen. 2. In hepatocytes from hyperthyroid rats maximal rates of ethanol oxidation were significantly lower than in hepatocytes from euthyroid rats, even though the capacity of the glycerol-3-phosphate cycle was increased. This was due to a decreased activity of alcohol dehydrogenase.
对正常甲状腺和甲状腺功能亢进大鼠肝细胞中苹果酸-天冬氨酸循环和3-磷酸甘油循环在乙醇氧化过程中对还原当量从细胞质转运到线粒体的贡献进行了定量研究。1. 在正常甲状腺大鼠的肝细胞中，两个循环的能力几乎相等，它们对线粒体总氢转运的相对贡献取决于所选择的条件。2. 在甲状腺功能亢进大鼠的肝细胞中，尽管3-磷酸甘油循环的能力增加，但乙醇氧化的最大速率显著低于正常甲状腺大鼠的肝细胞。这是由于乙醇脱氢酶活性降低所致。
