环丝氨酸对分离的大鼠肝细胞中线粒体和胞质天冬氨酸氨基转移酶的抑制作用。
Inhibition by cycloserine of mitochondrial and cytosolic aspartate aminotransferase in isolated rat hepatocytes.
作者信息
Janski A M, Cornell N W
出版信息
Biochem J. 1981 Mar 15;194(3):1027-30. doi: 10.1042/bj1941027.
Abstract
Isolated hepatocytes were incubated with L-cycloserine and then treated with digitonin so that mitochondrial and cytosolic fractions were obtained in 5 s. Mitochondrial and total cellular aspartate aminotransferases (EC 2.6.1.1) were inactivated in parallel. The enzyme was also inhibited in isolated mitochondria incubated with L-cycloserine. These results, in contrast with previous reports, indicate that cycloserine reacts equally with mitochondrial and cytosolic aspartate aminotransferases.
摘要
将分离的肝细胞与L-环丝氨酸一起孵育，然后用洋地黄皂苷处理，以便在5秒内获得线粒体和胞质部分。线粒体和全细胞天冬氨酸转氨酶（EC 2.6.1.1）同时失活。在与L-环丝氨酸孵育的分离线粒体中，该酶也受到抑制。与先前的报道相反，这些结果表明环丝氨酸与线粒体和胞质天冬氨酸转氨酶的反应相同。
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