显微注射N-乙基马来酰亚胺修饰的重酶解肌球蛋白对两栖类卵细胞分裂的影响。
Effect of microinjected N-ethylmaleimide-modified heavy meromyosin on cell division in amphibian eggs.
作者信息
Meeusen R L, Bennett J, Cande W Z
出版信息
J Cell Biol. 1980 Sep;86(3):858-65. doi: 10.1083/jcb.86.3.858.
Abstract
N-Ethylmaleimide-modified heavy meromyosin (NEM-HMM) microinjected into amphibian eggs inhibits cytokinesis and the cortical contractions associated with wound closure. Injection of NEM-HMM into two-cell Rana pipiens embryos produces a zone of cleavage inhibition around the point of injection. Early furrows followed by time-lapse microcinematography are seen to slow and stop as they enter the NEM-HMM-injected zone. Arrested furrows slowly regress, leaving a large region of cytoplasm uncleaved. Few nuclei are found in these regions of cleavage inhibition. Wound closure is often inhibited by NEM-HMM, especially when this inhibitor is injected just beneath the egg cortex. We observe that the surface of an unfertilized Rana egg is covered with microvilli that disappear during the course of development. The surfaces of NEM-HMM-inhibited zones remain covered with microvilli and resemble the unfertilized egg surface.
摘要
将N - 乙基马来酰亚胺修饰的重酶解肌球蛋白（NEM - HMM）显微注射到两栖类卵中会抑制胞质分裂以及与伤口愈合相关的皮质收缩。将NEM - HMM注射到双细胞豹蛙胚胎中，在注射点周围会产生一个卵裂抑制区。通过延时显微电影摄影术可以看到，早期的卵裂沟在进入注射了NEM - HMM的区域时会减慢并停止。停滞的卵裂沟会慢慢退回，留下一大片未分裂的细胞质区域。在这些卵裂抑制区域中很少发现细胞核。伤口愈合常常会被NEM - HMM抑制，尤其是当这种抑制剂注射在卵皮质下方时。我们观察到未受精的豹蛙卵表面覆盖着微绒毛，这些微绒毛在发育过程中会消失。NEM - HMM抑制区的表面仍然覆盖着微绒毛，类似于未受精卵的表面。
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