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The generation of haemolytic plaque-forming cells from human tonsils.

作者信息

Mawle A C

出版信息

Int Arch Allergy Appl Immunol. 1980;63(2):190-4. doi: 10.1159/000232625.

DOI:10.1159/000232625
PMID:6998875
Abstract

At low cell numbers (2 X 10(6) cells/culture) good numbers of plaque-forming cells (PFC) were obtained in response to stimulation by pokeweed mitogen (PWM). Maximum PFC were obtained with the Marbrook chamber using 2 X 10(6) cells/culture, stimulating cells with a 1:50 dilution of PWM and harvesting after 6--7 days. 20 different experiments using standard conditions gave a geometric mean of 646 PFC/10(6) initial cells. All PFC detected were IgM.

摘要

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