Andersen K B, von Meyenburg K
J Bacteriol. 1980 Oct;144(1):114-23. doi: 10.1128/jb.144.1.114-123.1980.
Batch cultures of Escherichia coli were grown in minimal media supplemented with various carbon sources which supported growth at specific growth rates from 0.2 to 1.3/h. The respiration rates of the cultures were measured continuously. With few exceptions, the specific rate of oxygen consumption was about 20 mmol of O2/h per g (dry weight), suggesting that the respiratory capacity was limited at this value. The adenosine triphosphate (ATP) required for the production of cell material from the different carbon sources was calculated on the basis of known ATP requirements in the biochemical pathways and routes of macromolecular synthesis. The calculated ATP requirements, together with the measured growth rates and growth yields on the different carbon sources, were used to calculate the rate of ATP synthesis by oxidative phosphorylation. This rate was closely related to the respiration rate. We suggest that aerobic growth of E. coli in batch cultures is limited by the rate of respiration and the concomitant rate of ATP generation through oxidative phosphorylation.
将大肠杆菌进行分批培养，培养于添加了各种碳源的基本培养基中，这些碳源能支持细菌以0.2至1.3 /小时的特定生长速率生长。持续测量培养物的呼吸速率。除少数例外情况，氧气消耗的比速率约为每克（干重）20 mmol O₂/小时，这表明呼吸能力在此值时受到限制。根据生物化学途径和大分子合成途径中已知的ATP需求，计算了利用不同碳源产生细胞物质所需的三磷酸腺苷（ATP）。计算得到的ATP需求，连同在不同碳源上测得的生长速率和生长产量，用于计算通过氧化磷酸化产生ATP的速率。该速率与呼吸速率密切相关。我们认为，分批培养中大肠杆菌的有氧生长受呼吸速率以及通过氧化磷酸化伴随产生ATP的速率限制。
