Mainzer S E, Hempfling W P
J Bacteriol. 1976 Apr;126(1):251-6. doi: 10.1128/jb.126.1.251-256.1976.
The effects of growth temperature on the aerobic growth yield with respect to oxygen consumption (Y0-grams [dry weight] per gram-atom of O) and the rate of maintenance respiration (m0-milligram-atoms of O/gram [dry weight] per hour) are reported for Escherichia coli B cultivated continuously in the presence of oxygen with limiting glucose. During anaerobic continuous culture, YATP(max) (grams [dry weight] per mole of ATP corrected for maintenance) increases from 10.3 to 12.7 as the growth temperature is lowered from 37 to 25 C. Over this same range, Y0(max) (Y0 corrected for maintenance respiration) rises from 12.5 to 28.8 and remains at the higher value down to 17.5 C. From 37 to 32 C, m0 increases from 0.9 to 4.4 but then falls to 1.5 as the temperature is lowered to 17.5 C. The value of m0 sharply rises some 13-fold as the temperature is raised to 42 C without a significant change in the value of Y0(max). Changes of Y0(max) are consistent with a temperature-sensitive doubling of the efficiency of oxidative phosphorylation, but the reasons for the changes of the rate of maintenance respiration are not known.
报道了在葡萄糖受限且有氧的条件下，连续培养的大肠杆菌B的生长温度对基于氧气消耗的有氧生长产量（Y0，每克原子氧对应的克数[干重]）和维持呼吸速率（m0，每小时每克[干重]的毫克原子氧）的影响。在厌氧连续培养过程中，随着生长温度从37℃降至25℃，YATP(max)（校正维持后的每摩尔ATP对应的克数[干重]）从10.3增加到12.7。在相同温度范围内，Y0(max)（校正维持呼吸后的Y0）从12.5升至28.8，并在降至17.5℃时保持较高值。从37℃到32℃，m0从0.9增加到4.4，但随着温度降至17.5℃，m0又降至1.5。当温度升至42℃时，m0的值急剧上升约13倍，而Y0(max)的值没有显著变化。Y0(max)的变化与氧化磷酸化效率的温度敏感性加倍一致，但维持呼吸速率变化的原因尚不清楚。
