Sloane J P, Ormerod M G, Imrie S F, Coombes R C
Br J Cancer. 1980 Sep;42(3):392-8. doi: 10.1038/bjc.1980.250.
We have investigated, by immunocytochemical means, the value of an antiserum raised to milk-fat-globule membranes in detecting metastatic deposits of breast carcinoma in conventional histological sections of liver, lymph nodes and marrow. The antiseum recognizes a membrane component, which we have called Epithelial Membrane Antigen, and which is confined to but widely distributed in epithelial tissues and tumours derived from them. In the sections examined, a greater extent of tumour infiltration was usually found, largely due to the identification of single malignant cells which normally go unrecognized with conventional stains. The number of positive samples was only increased, however, in sections of marrow aspirates, and the reasons for this are discussed. We suggest that further increases in detection rates could be attained by using the antiserum on cytological smears of marrow or even in cell suspensions.
我们通过免疫细胞化学方法研究了一种针对乳脂肪球膜产生的抗血清，在检测肝、淋巴结和骨髓的常规组织学切片中乳腺癌转移灶方面的价值。该抗血清识别一种膜成分，我们将其称为上皮膜抗原，它局限于上皮组织及其衍生的肿瘤中，但分布广泛。在所检查的切片中，通常发现肿瘤浸润范围更大，这主要是由于识别出了单个恶性细胞，而这些细胞用传统染色方法通常无法识别。然而，只有骨髓穿刺涂片的阳性样本数量增加了，对此原因进行了讨论。我们认为，通过在骨髓细胞学涂片甚至细胞悬液中使用该抗血清，检测率可能会进一步提高。
