Dearnaley D P, Ormerod M G, Sloane J P, Lumley H, Imrie S, Jones M, Coombes R C, Neville A M
J R Soc Med. 1983 May;76(5):359-64. doi: 10.1177/014107688307600506.
Single cells from mammary carcinoma infiltrating bone marrow can be detected in marrow aspirates using immunocytochemical stains for epithelial membrane antigen (EMA). This technique has been used to examine marrow aspirates taken from multiple sites from 24 patients at surgery for breast cancer. Ten of these patients had EMA-positive cells in their marrow, while 32 marrow samples from patients who did not have carcinoma were negative. These results have been combined with those obtained by taking aspirates from single sites from 47 breast patients without known skeletal deposits. Follow up showed that the patients with EMA-positive cells in their marrow developed bone metastases at a significantly faster rate.
使用上皮膜抗原（EMA）免疫细胞化学染色法，可在骨髓穿刺物中检测到浸润骨髓的乳腺癌单细胞。该技术已用于检查24例乳腺癌手术患者多个部位的骨髓穿刺物。其中10例患者的骨髓中有EMA阳性细胞，而32例无癌患者的骨髓样本为阴性。这些结果与47例无已知骨骼转移的乳腺癌患者单部位穿刺物的结果相结合。随访显示，骨髓中含有EMA阳性细胞的患者发生骨转移的速度明显更快。
