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迈向宫颈肿瘤定量细胞学筛查的自动化程序

Towards an automated procedure for the quantitative cytological screening of cervical neoplasms.

作者信息

Sincock A M, Middleton J, Moncrieff D

出版信息

J Clin Pathol. 1983 May;36(5):535-8. doi: 10.1136/jcp.36.5.535.

DOI:10.1136/jcp.36.5.535
PMID:6841647
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC498281/
Abstract

Integrating microdensitometry has been used to quantify the glycoprotein, epithelial membrane antigen (EMA), which is frequently expressed on the cytoplasmic membrane of neoplastic cells of the cervix uteri. The technique was able to identify and quantify the antigen over a wide concentration range and could also be semi-automated using a microcomputer interfaced to the microdensitometer and programmed with the simple high level language BASIC.

摘要

积分显微密度测定法已被用于定量糖蛋白，即上皮膜抗原（EMA），该抗原常在子宫颈肿瘤细胞的细胞质膜上表达。这项技术能够在很宽的浓度范围内识别和定量该抗原，并且还可以使用与显微密度计相连的微型计算机并通过简单的高级语言BASIC进行编程来实现半自动化。

相似文献

1
Towards an automated procedure for the quantitative cytological screening of cervical neoplasms.迈向宫颈肿瘤定量细胞学筛查的自动化程序
J Clin Pathol. 1983 May;36(5):535-8. doi: 10.1136/jcp.36.5.535.
2
Tumor marker studies of cervical smears. Potential for automation.宫颈涂片的肿瘤标志物研究。自动化潜力。
Acta Cytol. 1984 Jul-Aug;28(4):407-10.
3
A semi-automated procedure for aiding the diagnosis of cervical neoplasms based on the measurement of acid-labile DNA in exfoliated cells.一种基于测量脱落细胞中酸不稳定DNA辅助诊断宫颈肿瘤的半自动程序。
Int J Cancer. 1983 Jun 15;31(6):733-6. doi: 10.1002/ijc.2910310610.
4
Immunocytochemical staining of cervical smears for the diagnosis of cervical intraepithelial neoplasia.宫颈涂片的免疫细胞化学染色用于诊断宫颈上皮内瘤变。
Anal Quant Cytol. 1984 Sep;6(3):201-5.
5
Epithelial membrane antigen in cells from the uterine cervix: immunocytochemical staining of cervical smears.子宫颈细胞中的上皮膜抗原：宫颈涂片的免疫细胞化学染色
J Clin Pathol. 1984 Sep;37(9):984-9. doi: 10.1136/jcp.37.9.984.
6
Automated screening of cervical smears using immunocytochemical staining: a possible approach.使用免疫细胞化学染色对宫颈涂片进行自动筛查：一种可能的方法。
J Clin Pathol. 1985 Aug;38(8):886-92. doi: 10.1136/jcp.38.8.886.
7
[An immunohistochemical study of the distribution of epithelial membrane antigen (EMA) in uterine carcinoma].
Nihon Sanka Fujinka Gakkai Zasshi. 1987 May;39(5):739-45.
8
Fluorescent studies directed towards the location of abnormal epithelial cells in cervical smears.针对宫颈涂片异常上皮细胞定位的荧光研究。
Cytopathology. 1990;1(4):217-22. doi: 10.1111/j.1365-2303.1990.tb00350.x.
9
Expression of epithelial membrane and 3-fucosyl-N-acetyllactosamine antigens in cervix uteri with particular reference to adenocarcinoma in situ.子宫颈上皮膜抗原和3-岩藻糖基-N-乙酰乳糖胺抗原的表达，特别涉及原位腺癌。
J Clin Pathol. 1988 May;41(5):547-52. doi: 10.1136/jcp.41.5.547.
10
Semi-automated diagnosis of cervical intra-epithelial neoplasia grade 2 by the measurement of acid labile DNA in cytologically normal nuclei.通过测量细胞学正常细胞核中酸不稳定DNA对宫颈上皮内瘤变2级进行半自动诊断。
Cancer. 1986 Jul 1;58(1):83-6. doi: 10.1002/1097-0142(19860701)58:1<83::aid-cncr2820580115>3.0.co;2-j.

引用本文的文献

1
Microcomputer control applied to the Vickers M85/86 microdensitometer.微机控制应用于维氏M85/86显微密度计。
Histochem J. 1984 Jul;16(7):709-20. doi: 10.1007/BF01095277.
2
Epithelial membrane antigen in cells from the uterine cervix: immunocytochemical staining of cervical smears.子宫颈细胞中的上皮膜抗原：宫颈涂片的免疫细胞化学染色
J Clin Pathol. 1984 Sep;37(9):984-9. doi: 10.1136/jcp.37.9.984.
3
Demonstration of an epitope of the transferrin receptor in human cervical epithelium--a potentially useful cell marker.人宫颈上皮中转铁蛋白受体表位的显示——一种潜在有用的细胞标志物。
J Clin Pathol. 1984 Feb;37(2):131-5. doi: 10.1136/jcp.37.2.131.
4
Glutathione S-transferase (placental) as a marker of transformation in the human cervix uteri: an immunohistochemical study.谷胱甘肽S-转移酶（胎盘型）作为人子宫颈转化的标志物：一项免疫组织化学研究。
Br J Cancer. 1990 Oct;62(4):614-8. doi: 10.1038/bjc.1990.340.
5
Immunohistochemical and immunocytochemical study of bladder carcinomas using the epithelium-specific, tumour-associated monoclonal antibodies HMFG1 and AUA1.使用上皮特异性、肿瘤相关单克隆抗体HMFG1和AUA1对膀胱癌进行免疫组织化学和免疫细胞化学研究。
Br J Cancer Suppl. 1990 Jul;10:52-6.

本文引用的文献

1
Simultaneous Feulgen densitometry and autoradiographic grain counting with the Quantimet 720D image-analysis system. I. Estimation of nuclear DNA content in 3H-thymidine-labeled cells.使用Quantimet 720D图像分析系统同时进行福尔根密度测定和放射自显影片颗粒计数。I. 3H-胸腺嘧啶核苷标记细胞中核DNA含量的估计
J Histochem Cytochem. 1982 Jan;30(1):35-48. doi: 10.1177/30.1.7054272.
2
An immunocytochemical study of the distribution of epithelial membrane antigen in normal and disordered squamous epithelium.一项关于上皮膜抗原在正常及病变鳞状上皮中分布的免疫细胞化学研究。
Diagn Histopathol. 1982 Jan-Mar;5(1):11-7.
3
Use of antisera to epithelial membrane antigen for the cytodiagnosis of malignancy in serous effusions.使用抗上皮膜抗原血清进行浆液性积液中恶性肿瘤的细胞诊断。
J Clin Pathol. 1981 Dec;34(12):1326-32. doi: 10.1136/jcp.34.12.1326.
4
Increased detection of mammary carcinoma cells in marrow smears using antisera to epithelial membrane antigen.使用抗上皮膜抗原抗血清增加骨髓涂片中乳腺癌细胞的检测。
Br J Cancer. 1981 Jul;44(1):85-90. doi: 10.1038/bjc.1981.152.
5
Simple adaptations to extend the range of flow cytometry five orders of magnitude for the DNA analysis of uni-and multicellular systems.简单的适应性调整可将流式细胞术的范围扩展五个数量级，用于单细胞和多细胞系统的DNA分析。
J Histochem Cytochem. 1981 Jun;29(6):771-4. doi: 10.1177/29.6.7019314.
6
The use of antisera to epithelial membrane antigen in detecting micrometastases in histological sections.使用抗上皮膜抗原抗血清检测组织切片中的微转移灶。
Br J Cancer. 1980 Sep;42(3):392-8. doi: 10.1038/bjc.1980.250.
7
Feulgen-hydrolysis profiles in cells exfoliated from the cervix uteri: a potential aid in the diagnosis of malignancy.子宫颈脱落细胞中的福尔根水解图谱：对恶性肿瘤诊断的潜在辅助手段。
J Clin Pathol. 1982 Mar;35(3):345-9. doi: 10.1136/jcp.35.3.345.
8
Distribution of epithelial membrane antigen in normal and neoplastic tissues and it value in diagnostic tumor pathology.上皮膜抗原在正常组织和肿瘤组织中的分布及其在肿瘤诊断病理学中的价值。
Cancer. 1981 Apr 1;47(7):1786-95. doi: 10.1002/1097-0142(19810401)47:7<1786::aid-cncr2820470711>3.0.co;2-8.
9
Boeck's sarcoidosis of the breast: cytologic findings with aspiration biopsy cytology. A case clinically mimicking carcinoma.
Acta Cytol. 1978 Jan-Feb;22(1):1-2.
10
Applications of microcomputer technology to cytophotometry.微型计算机技术在细胞光度测定中的应用。
J Histochem Cytochem. 1979 Oct;27(10):1384-7. doi: 10.1177/27.10.92497.