Pessina A, Brambilla P, Mocarelli P
Biomedicine. 1980 Jul-Aug;33(4):105-9.
Ehrlich Ascites Tumor (EAT) may be regarded as an extreme example of a reduced expression of normal histocompatibility antigens which is also reflected in its ability to grow in almost any mouse strain. In this report we discuss some evidence about a specific antigenicity detected on the cell surface of EAT strain maintained by serial transfer in C57BL/6 mice. The antiserum against EAT antigen was prepared by immunizing rabbits with an extract obtained by KCl treatment of EAT cells and then tested for cytotoxicity on EAT cells. The specificity, confirmed by using the indirect immunofluorescence test and the inhibition of cytotoxicity by EAT antigen, was also discussed.
艾氏腹水瘤（EAT）可被视为正常组织相容性抗原表达降低的一个极端例子，这一点也体现在其几乎能在任何小鼠品系中生长的能力上。在本报告中，我们讨论了一些关于在C57BL/6小鼠中通过连续传代维持的EAT菌株细胞表面检测到的一种特异性抗原性的证据。通过用经KCl处理的EAT细胞提取物免疫兔子制备了抗EAT抗原的抗血清，然后检测其对EAT细胞的细胞毒性。还讨论了通过间接免疫荧光试验以及EAT抗原对细胞毒性的抑制作用所证实的特异性。
