Surface antigen on Ehrlich ascites tumor cells.

Ehrlich Ascites Tumor (EAT) may be regarded as an extreme example of a reduced expression of normal histocompatibility antigens which is also reflected in its ability to grow in almost any mouse strain. In this report we discuss some evidence about a specific antigenicity detected on the cell surface of EAT strain maintained by serial transfer in C57BL/6 mice. The antiserum against EAT antigen was prepared by immunizing rabbits with an extract obtained by KCl treatment of EAT cells and then tested for cytotoxicity on EAT cells. The specificity, confirmed by using the indirect immunofluorescence test and the inhibition of cytotoxicity by EAT antigen, was also discussed.