用于检测猪布鲁氏菌感染猪血清中抗体的酶联免疫吸附测定法的开发。
Development of an enzyme-linked immunosorbent assay for detecting antibodies in sera of Brucella suis-infected swine.
作者信息
Thoen C O, Hopkins M P, Armbrust A L, Angus R D, Pietz D E
出版信息
Can J Comp Med. 1980 Jul;44(3):294-8.
An enzyme-linked immunosorbent assay was developed using a heat-killed Brucella suis antigen for detecting antibodies in the sera of swine from which B. suis was isolated. Optimal enzyme-linked immunosorbent assay reactions were obtained using heat-killed B. suis antigen at a concentration comparable to McFarland Standard No. 1. Statistically significant differences were observed in the enzyme-linked immunosorbent assay results of 40 animals from which B. suis was isolated and the results for 48 noninfected swine at serum dilutions of 1:25 and 1:50 (P < 0.0001). The enzyme-linked immunosorbent assay is a rapid reproducible test which can be readily automated that appears to have practical value for screening large numbers of breeding and slaughter swine for brucellosis.
利用热灭活猪布鲁氏菌抗原开发了一种酶联免疫吸附测定法，用于检测分离出猪布鲁氏菌的猪血清中的抗体。使用浓度与麦氏比浊标准1号相当的热灭活猪布鲁氏菌抗原可获得最佳酶联免疫吸附测定反应。在血清稀释度为1:25和1:50时，观察到从40只分离出猪布鲁氏菌的动物的酶联免疫吸附测定结果与48只未感染猪的结果存在统计学显著差异（P < 0.0001）。酶联免疫吸附测定法是一种快速可重复的检测方法，易于自动化，对于大规模筛查种猪和屠宰猪的布鲁氏菌病似乎具有实用价值。
Zotero 插件