使用酶免疫测定法鉴定布鲁氏菌的A抗原和M抗原。
Use of an enzyme immunoassay test for characterizing the A and M antigens of Brucella.
作者信息
Thoen C O, Armbrust A L, Eacret W G, Harrington R, Brown G M
出版信息
J Clin Microbiol. 1979 Apr;9(4):485-7. doi: 10.1128/jcm.9.4.485-487.1979.
Abstract
An enzyme immunoassay test was developed for detecting the A and M antigens of brucellae. One hundred fifteen isolates, including 96 strains of Brucella abortus, 5 strains of B. suis, 1 strain of B. melitensis and 1 strain of B. neotomae, were accurately serotyped with the enzyme immunoassay test. No reactions were observed with 11 isolates of B. canis and 1 isolate of B. ovis. This rapid serological test provides for considerable savings in time and materials as compared with the standard tube agglutination test.
摘要
开发了一种酶免疫测定试验来检测布鲁氏菌的A和M抗原。用该酶免疫测定试验对115株分离菌进行了准确的血清分型，其中包括96株流产布鲁氏菌、5株猪布鲁氏菌、1株羊布鲁氏菌和1株墨西哥布鲁氏菌。对11株犬布鲁氏菌和1株绵羊布鲁氏菌的分离菌未观察到反应。与标准试管凝集试验相比，这种快速血清学试验可节省大量时间和材料。
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本文引用的文献
1
Peroxidase-labeled antibody. A new method of conjugation.
J Histochem Cytochem. 1974 Dec;22(12):1084-91. doi: 10.1177/22.12.1084.
2
The utilization of Brucella abortus culturing and biotyping results in the epizootiologic investigation of bovine brucellosis.
Proc Annu Meet U S Anim Health Assoc. 1973(77):85-99.
3
Laboratory summary of Brucella isolations and typing: 1975.
Am J Vet Res. 1976 Oct;37(10):1241-2.
4
Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test.
J Clin Microbiol. 1976 Jun;3(6):604-8. doi: 10.1128/jcm.3.6.604-608.1976.
5
An enzyme-labeled antibody test for detecting antibodies in chickens infected with Mycobacterium avium serotype 2.
Avian Dis. 1978 Jan-Mar;22(1):162-6.
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