Sabukunze Serges, Gu Haorong, Zhao Lin, Jia Honglin, Guo Huanping
State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Heilongjiang Research Center for Veterinary Biopharmaceutical Technology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.
Front Vet Sci. 2024 Jun 5;11:1423581. doi: 10.3389/fvets.2024.1423581. eCollection 2024.
Toxoplasmosis is an important zoonotic disease caused by that can infect almost all warm-blooded animals worldwide, including humans. The high prevalence of infection and its ability to cause serious harm to humans and animals, especially immunodeficient individuals, make it a key public health issue. Accurate diagnostic tools with high sensitivity are needed for controlling infection. In the current study, we compared the performance of recombinant SAG2, GRA6, and GRA7 in ELISA for the serological diagnosis of infection in cats. We further investigated the antigenicity of recombinant dense granule protein 3 (rGRA3), rGRA5, rGRA8, and rSRS29A expressed in a plant-based, cell-free expression system for detecting antibodies in -infected cats. In summary, our data suggest that GRA7 is more sensitive than the other two antigens for the serodiagnosis of infection in cats, and GRA3 expressed in the cell-free system is also a priming antigen in serological tests for detecting infection in cats.
弓形虫病是一种由[病原体名称缺失]引起的重要人畜共患病，可感染全球几乎所有温血动物，包括人类。[病原体名称缺失]感染的高流行率及其对人类和动物，尤其是免疫功能低下个体造成严重危害的能力，使其成为一个关键的公共卫生问题。控制[病原体名称缺失]感染需要高灵敏度的准确诊断工具。在本研究中，我们比较了重组SAG2、GRA6和GRA7在酶联免疫吸附测定（ELISA）中对猫弓形虫感染进行血清学诊断的性能。我们进一步研究了在基于植物的无细胞表达系统中表达的重组致密颗粒蛋白3（rGRA3）、rGRA5、rGRA8和rSRS29A用于检测弓形虫感染猫体内抗体的抗原性。总之，我们的数据表明，GRA7在猫弓形虫感染血清学诊断中比其他两种抗原更敏感，并且在无细胞系统中表达的GRA3也是猫弓形虫感染血清学检测中的一种引发抗原。
