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弓形虫病中的间接免疫荧光:极性染色的频率、性质和特异性。

Indirect immunofluorescence in toxoplasmosis: frequency, nature and specificity of polar staining.

作者信息

van Renterghem L, van Nimmen L

出版信息

Zentralbl Bakteriol Orig A. 1976 Aug;235(4):559-65.

PMID:790860
Abstract

In the indirect immunofluorescence test ant the Remington test for Toxoplasmosis of some sera, a polar fluorescence of the parasite has been observed. This "Polar Factor" is not observed in umbilical cord blood and in sera of children less than 6 months old, but has a relative high frequency in sera of adults. The detection of the factor depends on the nature of the used antigen. With sucrose gradient centrifugation, anti-IgM immunofluorescence, 2-mercaptoethanol-treatment and selective adsorption of sera, we could demonstrate that this factor is a non-specific IgM.

摘要

在对某些血清进行弓形虫病的间接免疫荧光试验和雷明顿试验中,观察到寄生虫的极性荧光。这种“极性因子”在脐带血和6个月以下儿童的血清中未观察到,但在成人血清中的出现频率相对较高。该因子的检测取决于所用抗原的性质。通过蔗糖梯度离心、抗IgM免疫荧光、2-巯基乙醇处理和血清的选择性吸附,我们可以证明该因子是一种非特异性IgM。

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1
Indirect immunofluorescence in toxoplasmosis: frequency, nature and specificity of polar staining.弓形虫病中的间接免疫荧光:极性染色的频率、性质和特异性。
Zentralbl Bakteriol Orig A. 1976 Aug;235(4):559-65.
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[Use of the ELISA (enzyme-linked immunosorbent assay) method in detection of anti-toxoplasma IgG and IgM].[酶联免疫吸附测定(ELISA)法在检测抗弓形虫IgG和IgM中的应用]
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Detection of IgM-anti-toxoplasma antibodies in acute acquired and congenital toxoplasmosis.急性获得性和先天性弓形虫病中IgM抗弓形虫抗体的检测
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A solid-phase indirect haemadsorption assay (SPIHA) for detection of immunoglobulin m antibodies to Toxoplasma gondii: application to diagnosis of acute acquired toxoplasmosis.用于检测抗弓形虫免疫球蛋白M抗体的固相间接血吸附试验(SPIHA):在急性获得性弓形虫病诊断中的应用
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Direct agglutination test for diagnosis of Toxoplasma infection: method for increasing sensitivity and specificity.
用于诊断弓形虫感染的直接凝集试验:提高敏感性和特异性的方法
J Clin Microbiol. 1980 Jun;11(6):562-8. doi: 10.1128/jcm.11.6.562-568.1980.
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J Clin Microbiol. 1980 Dec;12(6):780-4. doi: 10.1128/jcm.12.6.780-784.1980.
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Multiple-antigen slide test for detection of immunoglobulin M antibodies in newborn and infant sera by immunofluorescence.用于通过免疫荧光检测新生儿和婴儿血清中免疫球蛋白M抗体的多抗原玻片试验。
J Clin Microbiol. 1981 Apr;13(4):631-6. doi: 10.1128/jcm.13.4.631-636.1981.
6
Toxoplasma antigens recognized by naturally occurring human antibodies.被天然存在的人体抗体识别的弓形虫抗原。
J Clin Microbiol. 1986 Dec;24(6):1050-4. doi: 10.1128/jcm.24.6.1050-1054.1986.
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J Clin Microbiol. 1988 Nov;26(11):2444-6. doi: 10.1128/jcm.26.11.2444-2446.1988.
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Application of quantitative immunofluorescence to clinical serology: antibody levels of Toxoplasma gondii.定量免疫荧光在临床血清学中的应用:弓形虫抗体水平
J Clin Microbiol. 1989 Sep;27(9):2008-13. doi: 10.1128/jcm.27.9.2008-2013.1989.
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