用于拉沙病毒的红细胞-球蛋白结合物在反向被动血凝及抑制试验中的制备与应用
Preparation and use of erythrocyte-globulin conjugates to Lassa virus in reversed passive hemagglutination and inhibition.
作者信息
Goldwasser R A, Elliott L H, Johnson K M
出版信息
J Clin Microbiol. 1980 Jun;11(6):593-9. doi: 10.1128/jcm.11.6.593-599.1980.
Conditions were defined for functional covalent coupling of anti-Lassa virus globulins to glutaraldehyde-fixed chicken erythrocytes. Tolylene-2,4-diisocyanate in a reaction mixture containing not more than 0.01 M NaCl produced uniformly good conjugates which were used in reversed passive hemagglutination (RPH) and reversed passive hemagglutination inhibition (RPHI) tests to detect Lassa virus antigens in infected cell cultures and specific antigens in Vero cell cultures. Identical results were obtained with this method and with immunofluorescent-antibody (IFA) staining in the detection and identification of Lassa virus isolated from human and rodent specimens from West Africa. The RPHI method was equal to IFA for serological diagnosis of acute human Lassa virus infection and superior to IFA, complement fixation, and a radioimmunoassay procedure for detection of Lassa virus antibodies in a human population where this infection is endemic.
确定了将抗拉沙病毒球蛋白与戊二醛固定的鸡红细胞进行功能性共价偶联的条件。在含有不超过0.01M氯化钠的反应混合物中，甲苯-2,4-二异氰酸酯产生了均匀良好的缀合物，这些缀合物用于反向被动血凝（RPH）和反向被动血凝抑制（RPHI）试验，以检测感染细胞培养物中的拉沙病毒抗原和Vero细胞培养物中的特异性抗原。用该方法与免疫荧光抗体（IFA）染色法从西非的人类和啮齿动物标本中检测和鉴定拉沙病毒时，得到了相同的结果。RPHI方法在急性人类拉沙病毒感染的血清学诊断方面与IFA相当，在检测该感染流行地区人群中的拉沙病毒抗体方面优于IFA、补体结合试验和放射免疫测定法。
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