多价病毒免疫荧光细胞内抗原的制备及其在人类血清学调查中的应用。
Preparation of polyvalent viral immunofluorescent intracellular antigens and use in human serosurveys.
作者信息
Johnson K M, Elliott L H, Heymann D L
出版信息
J Clin Microbiol. 1981 Nov;14(5):527-9. doi: 10.1128/jcm.14.5.527-529.1981.
Abstract
A method is described for preparation of polyvalent antigens for use in rapid screening for immunofluorescent antibodies to Lassa, Marburg, and Ebola viruses. The technique uses mixtures of specifically infected Vero cells placed on Teflon-templated microscopy slides. It was found to be as sensitive as the use of monovalent antigens for detection and quantitation of antibodies to these highly hazardous human pathogens.
摘要
描述了一种制备多价抗原的方法，用于快速筛选针对拉沙病毒、马尔堡病毒和埃博拉病毒的免疫荧光抗体。该技术使用放置在聚四氟乙烯模板显微镜载玻片上的特异性感染的Vero细胞混合物。结果发现，在检测和定量针对这些高度危险的人类病原体的抗体方面，该方法与使用单价抗原一样敏感。
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