Johnson H M, Brenner K, Angelotti R, Hall H E
J Bacteriol. 1966 Mar;91(3):967-74. doi: 10.1128/jb.91.3.967-974.1966.
Johnson, H. M. (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), K. Brenner, R. Angelotti, and H. E. Hall. Serological studies of types A, B, and E botulinal toxins by passive hemagglutination and bentonite flocculation. J. Bacteriol. 91:967-974. 1966.-Formalinized sheep red blood cells (SRBC), sensitized with types A, B, and E botulinal toxoids and toxins by bis-diazotized benzidine (BDB), were tested against A, B, and E antitoxins prepared in horses and rabbits. Type B antitoxin cross-reacted with A toxoid SRBC, but the reciprocal cross-reaction was not observed. E toxin SRBC were specifically agglutinated by E antitoxin. Flocculation of antigen-sensitized bentonite particles was less sensitive in titration of antitoxin than hemagglutination. Also, reciprocal cross-reactions were observed between types A and B antitoxins. Cross-reactions in both serological tests were eliminated by titration of antitoxins in the presence of the heterologous antigens, with no inhibitory effect on the homologous antitoxins. Generally, equine antitoxins were less suitable for agglutinations, especially of antigen-sensitized bentonite particles. Types A, B, and E antitoxins were specifically inhibited by 43, 39, and 245 mouse ld(50) of their respective homologous toxins in the hemagglutination-inhibition test. A, B, and E antitoxins were specifically inhibited by 500, 950, and 1,500 mouse ld(50) of their respective homologous toxins in bentonite flocculation inhibitions. Formalinized SRBC sensitized with rabbit types A and B antitoxins by BDB were respectively clumped by as little as 0.75 to 1.3 mouse ld(50) of A toxin and 2.3 ld(50) of B toxin, whereas bentonite particles sensitized by the same antitoxins were specifically clumped by 150 ld(50) of A toxin and 630 ld(50) of B toxin. E antitoxin sensitization of SRBC or bentonite particles was not successful. Evidence is presented that indicates that the serological procedures are applicable to the detection of botulinal toxins in food.
约翰逊，H. M.（俄亥俄州辛辛那提市罗伯特·A. 塔夫脱卫生工程中心），K. 布伦纳，R. 安杰洛蒂，以及H. E. 霍尔。通过被动血凝和膨润土絮凝对A、B和E型肉毒杆菌毒素进行血清学研究。《细菌学杂志》91:967 - 974。1966年。——用双偶氮联苯胺（BDB）使A、B和E型肉毒类毒素及毒素致敏的甲醛化绵羊红细胞（SRBC），与在马和兔体内制备的A、B和E型抗毒素进行检测。B型抗毒素与A型类毒素SRBC发生交叉反应，但未观察到反向交叉反应。E毒素SRBC被E型抗毒素特异性凝集。在抗毒素滴定中，抗原致敏的膨润土颗粒的絮凝比血凝不敏感。此外，在A和B型抗毒素之间观察到反向交叉反应。在异源抗原存在下对抗毒素进行滴定可消除两种血清学试验中的交叉反应，且对同源抗毒素无抑制作用。一般来说，马抗毒素不太适合用于凝集，尤其是对抗原致敏的膨润土颗粒。在血凝抑制试验中，A、B和E型抗毒素分别被其各自同源毒素的43、39和245小鼠半数致死量（ld50）特异性抑制。在膨润土絮凝抑制试验中，A、B和E型抗毒素分别被其各自同源毒素的500、950和1500小鼠ld50特异性抑制。用BDB使兔A和B型抗毒素致敏的甲醛化SRBC，分别被低至0.75至1.3小鼠ld50的A型毒素和2.3 ld50的B型毒素凝集，而用相同抗毒素致敏的膨润土颗粒分别被150 ld50的A型毒素和630 ld50的B型毒素特异性凝集。用E型抗毒素致敏SRBC或膨润土颗粒未成功。有证据表明这些血清学方法适用于检测食品中的肉毒杆菌毒素。
