Schmidt J, Brunnemann H, Stelzner A, Süss J, Kittlick M, Oehring H
Z Erkr Atmungsorgane. 1980;154(1):18-35.
Considering mainly practical aspects, a review of available methods of virological diagnosis of acute respiratory diseases is given. The first part contains the elements of virological diagnostics, especially a description and valuation of rapid detection of virus using fluorescent antibodies, and of haemadsorption technique for rapid diagnosis. Moreover, methods of viral isolation (cell cultures, embryonated eggs), conventional serological tests (CFR, HAIT, NT), and recent, not yet generally applied serological techniques (passive haemagglutination, indirect fluorescent antibody technique, haemadsorption inhibition test, radial immunodiffusion, radial immunohaemolysis, radio immune assay, enzyme linked immune assay) are described and valued as diagnostic tools in viral diseases of respiratory system. The second part deals with the specific diagnostics of infections by influenza, parainfluenza, RS-, adeno-, corona-, and rhinoviruses. Special instructions for the application of available tests and for the interpretation of results are given.
主要从实际应用角度出发，对急性呼吸道疾病病毒学诊断的现有方法进行了综述。第一部分包含病毒学诊断的要素，特别是使用荧光抗体快速检测病毒以及用于快速诊断的血吸附技术的描述和评估。此外，还介绍了病毒分离方法（细胞培养、鸡胚）、传统血清学检测方法（补体结合反应、血凝抑制试验、中和试验）以及近期尚未普遍应用的血清学技术（被动血凝、间接荧光抗体技术、血吸附抑制试验、放射免疫扩散、放射免疫溶血、放射免疫测定、酶联免疫测定），并对其作为呼吸系统病毒疾病诊断工具的价值进行了评估。第二部分论述了流感病毒、副流感病毒、呼吸道合胞病毒、腺病毒、冠状病毒和鼻病毒感染的特异性诊断。给出了现有检测方法应用及结果解读的具体说明。
