改良API 20 C、Mycotube和Bacto-白色念珠菌抗血清在常规实验室中用于酵母鉴定的适用性。

Suitability of the modified API 20 C, Mycotube and Bacto-Candida-albicans-Antiserum for the identification of yeasts in the routine laboratory.

作者信息

Dickgiesser N, Pieringer E

出版信息

Zentralbl Bakteriol A. 1980 Jun;247(1):132-7. doi: 10.1016/s0172-5599(80)80029-0.

Abstract

In a clinical routine study 304 yeasts were examined with the aid of API 20 C Auxanogram (Analytab Products Inc.) Mycotube (Roche) and Bacto-Candida-albicans-Antiserum (Difco) (=CAA). API (in parenthesis the result of the Mycotube test) identified 99% (92.4%) correctly; without considering C. albicans, the result was correct in 96.5% (75.8%). The CAA indicated a high specificity against C. albicans. For the Routine Laboratory we recommend screening of all yeasts with the CAA; an additional morphological examination is also recommended as cross-reactions with C.tropicalis and Torulopsis species are found. The non-agglutinating yeasts can then be further examined with the API 20 C.

摘要

在一项临床常规研究中，借助API 20 C Auxanogram（Analytab Products Inc.）、Mycotube（罗氏公司）和Bacto - 白色念珠菌抗血清（Difco）（=CAA）对304株酵母菌进行了检测。API（括号内为Mycotube试验结果）正确识别率为99%（92.4%）；不考虑白色念珠菌时，正确识别率为96.5%（75.8%）。CAA显示出对白色念珠菌的高特异性。对于常规实验室，我们建议用CAA对所有酵母菌进行筛查；由于发现与热带念珠菌和球拟酵母菌属存在交叉反应，还建议进行额外的形态学检查。然后可以用API 20 C对不凝集的酵母菌进行进一步检测。