Davies J, Jimenez A
Am J Trop Med Hyg. 1980 Sep;29(5 Suppl):1089-92. doi: 10.4269/ajtmh.1980.29.1089.
An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. A bacterial R-plasmid determinant that phosphorylates and inactivates antibiotic G418 can be introduced into yeast by transformation and expresses resistance to G418. It is suggested that this combination of antibiotic and dominant resistance mechanism may be useful in recombinant DNA studies as a cloning selection in eukaryotes.
氨基糖苷类抗生素G418已被证明在浓度为1至300微克/毫升时，是许多原核生物和真核生物的抑制剂。一种能使抗生素G418磷酸化并使其失活的细菌R质粒决定簇，可通过转化导入酵母，并表达对G418的抗性。有人认为，这种抗生素与显性抗性机制的组合，在重组DNA研究中作为真核生物的克隆选择可能是有用的。
