Inoue Y, Kobayashi S, Yoshikawa K, Tran L T, Kimura A
Research Institute for Food Science, Kyoto University, Japan.
Biotechnol Appl Biochem. 1993 Jun;17(3):305-10.
Two kinds of DNA fragments concerned with resistance against lipid hydroperoxide were cloned from the yeast Saccharomyces cerevisiae. When the recombinant plasmid carrying the DNA fragment was introduced into the cells of S. cerevisiae which do not have an appropriate auxotrophic marker, the transformants could be selected by their resistance to t-butyl hydroperoxide. By Southern hybridization analysis, such transformants were found to contain the recombinant plasmid, suggesting that the DNA fragment could be used as a selection marker for the transformation of S. cerevisiae.
