Morzlock F V
Growth. 1980 Jun;44(2):123-32.
Early bud and palette limb regenerates of the adult newt, Notophthalmus viridescens, were compared with respect to the effect of denervation on the activities of some of their hydrolases in order to determine if these enzymes contribute to the atrophy of denervated regenerates. Results show that denervation increased total activities of acid protease and acid phosphatase by 77-89% and 41-45% respectively at both stages examined. Both membrane-bound and soluble enzymatic forms contributed to the observed increase in the total activity of each hydrolase. Furthermore, the actual levels of each enzyme attained at the two stages following denervation were quite similar to each other. Since each hydrolase responded to denervation in a similar way at both stages examined, it was concluded that resorption of denervated limb regenerates is not primarily due to the increased activity of hydrolases. Denervated early bud regenerates, which are almost completely resorbed following nerve withdrawal, would be expected to show greater levels of hydrolytic activity than denervated palette regenerates, which exhibit only a partial resorption following the same operation.
为了确定某些水解酶是否导致去神经支配的再生肢体萎缩，对成年绿红东美螈早期芽和蹼状肢体再生进行了比较，研究去神经支配对其部分水解酶活性的影响。结果表明，在所检测的两个阶段，去神经支配分别使酸性蛋白酶和酸性磷酸酶的总活性提高了77 - 89%和41 - 45%。膜结合型和可溶性酶形式都导致了所观察到的每种水解酶总活性的增加。此外，去神经支配后两个阶段达到的每种酶的实际水平彼此非常相似。由于在所检测的两个阶段每种水解酶对去神经支配的反应相似，因此得出结论，去神经支配的肢体再生的吸收主要不是由于水解酶活性的增加。去神经支配的早期芽再生在神经去除后几乎完全被吸收，预计其水解活性水平会比去神经支配的蹼状再生肢体更高，后者在相同操作后仅表现出部分吸收。
