鲁米诺依赖性化学发光反应测量值与细菌吞噬易感性之间的相关性。
Correlation between measurements of the luminol-dependent chemiluminescence response and bacterial susceptibility to phagocytosis.
作者信息
Welch W D
出版信息
Infect Immun. 1980 Nov;30(2):370-4. doi: 10.1128/iai.30.2.370-374.1980.
Abstract
The generation of chemiluminescence by phagocytosing leukocytes has been suggested to reflect concomitant microbicidal activity. Correlation between measurements of the chemiluminescence response and susceptibility of bacteria to phagocytosis, however, has not been studied. To examine and compare a range of responses in the two assays, four Escherichia coli serotypes were chosen as test organisms with degrees of susceptibilities to phagocytosis ranging from 0 to 100% bacteria killed. No complete correlation between peak, slope, or curve area integral measurements of the chemiluminescence response and bacterial susceptibility to phagocytosis were found, although a correlation between the two assays could be made after using specific opsonization procedures like the addition of antiserum to selected serotypes. Intrinsic differences present among the bacterial serotypes may be responsible for the observed lack of correlation between the two assays.
摘要
吞噬白细胞产生化学发光被认为反映了伴随的杀菌活性。然而，化学发光反应测量与细菌吞噬易感性之间的相关性尚未得到研究。为了检查和比较两种检测方法中的一系列反应，选择了四种大肠杆菌血清型作为测试生物体，其吞噬易感性程度从0%到100%的细菌被杀灭。尽管在使用特定的调理程序（如向选定血清型添加抗血清）后可以建立两种检测方法之间的相关性，但在化学发光反应的峰值、斜率或曲线面积积分测量与细菌吞噬易感性之间未发现完全相关性。细菌血清型之间存在的内在差异可能是导致两种检测方法之间缺乏相关性的原因。
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