Yue B Y, Niedra R, Baum J L
Invest Ophthalmol Vis Sci. 1980 Dec;19(12):1471-6.
Rabbit corneal endothelial primary cultures and subcultures with initial high seeding densities (split ratio 1 : 2) and low seeding densities (split ratio 1 : 4) were maintained routinely in Falcon Petri dishes. Ascorbate, 75 microgram/ml, was added daily to experimental cultures to evaluate its effect on growth of endothelial cells. With supplemental ascorbic acid, the cells grew at a slow rate, had a shorter lifespan, and reached a lower cell density at confluency than did control cells. The inhibitory effect of ascorbic acid on cell growth was more pronounced with cultures plated at low cell densities and with cells in later passages. Cultures grown with ascorbate contained a greater number of elongated cells and large-sized cells with vacuoles. A basal lamina was observed even in control cultures with no supplemental ascorbate.
将初始高接种密度（传代比例1:2）和低接种密度（传代比例1:4）的兔角膜内皮原代培养物和传代培养物常规培养于Falcon培养皿中。每天向实验培养物中添加75微克/毫升的抗坏血酸盐，以评估其对内皮细胞生长的影响。添加补充抗坏血酸后，细胞生长速度缓慢，寿命较短，汇合时达到的细胞密度低于对照细胞。抗坏血酸对细胞生长的抑制作用在低细胞密度接种的培养物和后期传代的细胞中更为明显。用抗坏血酸盐培养的培养物中含有更多的伸长细胞和有液泡的大细胞。即使在未添加补充抗坏血酸盐的对照培养物中也观察到了基底层。
