Endrich B, Asaishi K, Götz A, Messmer K
Res Exp Med (Berl). 1980;177(2):125-34. doi: 10.1007/BF01851841.
An experimental model was designed for direct, quantitative studies of hemodynamic and morphologic parameters in the microcirculation. It consists of implanting a modified Algire chamber in the dorsal skin flap of hamsters and the implementation of two permanent catheters in jugular vein and carotid artery. The microcirculation was studied using intravital microscopy and television techniques for in situ measurements of blood cell velocity and vascular diameters. Due to the poor contrast between blood cells, blood capillaries and surrounding s.c. tissue, microvascular beds were visualized using fluorescent microscopy after i.v. injection of 0.2 ml of 5% FITC-Dextran 150. The combination of optical elements and low amounts of FITC-Dextran improved the contrast of the televised image without changing macro- and micro-hemodynamic parameters, and blood plasma was delineated as bright structure against the substantially darker background of red blood cells and surrounding tissue. This permitted the quantitative study of practically all blood vessels within a given field of s.c. tissue in unanesthetized animals. Blood cell velocity in arterioles was 0.7-1.1 mm/s, 0.2-0.7 mm/s in midcapillaries and reached 0.6 mm/s in collecting venules. Since i.v. injection of drugs and systemic pressure measurements are possible in this model, it provides a unique means for studying the reactivity of the microcirculation over a prolonged period.
设计了一种实验模型，用于直接定量研究微循环中的血流动力学和形态学参数。该模型包括在仓鼠背部皮瓣中植入改良的阿尔吉尔小室，并在颈静脉和颈动脉中插入两根永久性导管。使用活体显微镜和电视技术研究微循环，以原位测量血细胞速度和血管直径。由于血细胞、毛细血管与周围皮下组织之间的对比度较差，在静脉注射0.2 ml 5%异硫氰酸荧光素标记的葡聚糖150后，使用荧光显微镜观察微血管床。光学元件与少量异硫氰酸荧光素标记的葡聚糖相结合，在不改变宏观和微观血流动力学参数的情况下提高了电视图像的对比度，血浆在红细胞和周围组织的深得多的背景衬托下呈现为明亮结构。这使得在未麻醉动物的给定皮下组织区域内对几乎所有血管进行定量研究成为可能。微动脉中的血细胞速度为0.7-1.1毫米/秒，中间毛细血管中的速度为0.2-0.7毫米/秒，集合微静脉中的速度达到0.6毫米/秒。由于在该模型中可以进行静脉注射药物和测量全身压力，它为长时间研究微循环的反应性提供了一种独特的方法。
