Jókay I, Kelemenics K, Karczag E, Földes I
Immunobiology. 1980 Dec;157(4-5):390-400. doi: 10.1016/S0171-2985(80)80008-8.
Interactions of glucocorticoids and heparin on the humoral immune response of mice to SRBC have been studied. Heparin injected subcutaneously in a depot-form 2 hours before immunization restores the antibody-forming ability of mice immuno-suppressed by hydrocortisone, through pretreatment of mice only with heparin 3 days before immunization decreases the PFC-count in the spleen. Cortisone (ip) and heparin (sc) per se injected at about the time of immunization with suboptimal doses of SRBC resulted only in a slight stimulating or no effect on spleen PFC count and serum antibody level. Cortisone and heparin applied in combination, however, exerted a stimulatory effect on the humoral immune response to SRBC. The route of steroid administration pointed to the significance of macrophages in this phenomenon. As the cooperative effect of heparin + cortisone on the humoral immune response shows a good correlation with their cooperative effect on in vivo (3H)dThd incorporation and thymidine kinase activity of spleen, it was hypothesized that an in vivo mitogenic activity for the spleen cells was responsible for the adjuvant effect observed.
已对糖皮质激素和肝素对小鼠针对绵羊红细胞（SRBC）的体液免疫反应的相互作用进行了研究。在免疫前2小时以长效制剂形式皮下注射肝素，可恢复被氢化可的松免疫抑制的小鼠的抗体形成能力，而仅在免疫前3天用肝素预处理小鼠会降低脾脏中的空斑形成细胞（PFC）计数。在以次优剂量的SRBC免疫时注射可的松（腹腔注射）和肝素（皮下注射）本身，对脾脏PFC计数和血清抗体水平仅产生轻微刺激或无影响。然而，联合应用可的松和肝素对针对SRBC的体液免疫反应产生了刺激作用。类固醇给药途径表明巨噬细胞在这一现象中的重要性。由于肝素+可的松对体液免疫反应的协同作用与其对脾脏体内（3H）脱氧胸苷（dThd）掺入和胸苷激酶活性的协同作用具有良好的相关性，因此推测脾脏细胞的体内促有丝分裂活性是观察到的佐剂效应的原因。
