Karczag E, Kelemenics K, Jókay I, Földes I
Immunobiology. 1980 Dec;157(4-5):379-89. doi: 10.1016/S0171-2985(80)80007-6.
The effect of glucocorticoids and heparin (in depot form) on the DNA-synthesis of thymus and spleen of mice was investigated by measuring (3H)dThd incorporation in a four day period. The kinetics of glucocorticoid action was similar whether the hormones were applied in 11 beta-hydroxy-, or keto-form and was characterized by a suppressive effect with a maximum on the first two days after administration. The suppressive effect of glucocorticoids proved to be more marked in the thymus than in the spleen. Heparin per se did not change thymidine incorporation into the lymphoid organs, but injected 3 hours prior steroid administration inhibited the suppressive effect of cortisone, but not that of hydrocortisone. In addition, heparin even reversed the suppressing effect of cortisone on splenic (3H)dThd uptake. The effects of heparin on glucocorticoid action could be only observed when the steroid was applied intraperitoneally. This observation and other data discussed suggest the involvement of peritoneal cells (macrophages) in glucocorticoid-heparin interactions on DNA-synthesis of lymphoid organs.
通过在四天时间内测量（3H）dThd掺入量，研究了糖皮质激素和（长效剂型）肝素对小鼠胸腺和脾脏DNA合成的影响。无论激素以11β-羟基形式还是酮形式应用，糖皮质激素作用的动力学都是相似的，其特征是具有抑制作用，在给药后的头两天达到最大。糖皮质激素的抑制作用在胸腺中比在脾脏中更为明显。肝素本身不会改变胸腺嘧啶核苷掺入淋巴器官的情况，但在给予类固醇前3小时注射肝素可抑制可的松的抑制作用，但对氢化可的松的抑制作用无影响。此外，肝素甚至可逆转可的松对脾脏（3H）dThd摄取的抑制作用。只有当类固醇经腹腔注射时，才能观察到肝素对糖皮质激素作用的影响。这一观察结果以及所讨论的其他数据表明，腹膜细胞（巨噬细胞）参与了糖皮质激素-肝素对淋巴器官DNA合成的相互作用。
