Parker K, Vernadakis A
J Neurochem. 1980 Jul;35(1):155-63. doi: 10.1111/j.1471-4159.1980.tb12501.x.
Age-dependent decreases in the levels of ornithine decarboxylase activity were observed in the optic lobes, cerebral hemispheres, and midbrain-diencephalon of 6--17-day-old chick embryos. In dissociated cell cultures from chick embryonic brains a similar pattern of declining ornithine decarboxylase activity with time in culture was observed. Ornithine decarboxylase activity in the dissociated brain cell cultures was stimulated by changing the culture medium. The peak stimulatory effect was shown to occur 12 h after changing the medium. Although serum-free medium stimulated ornithine decarboxylase activity slightly, the presence of serum in the medium was the primary stimulatory factor. Both fetal calf serum and heat-inactivated fetal calf serum produced dose-dependent stimulation of ornithine decarboxylase activity. Dialyzed fetal calf sera stimulated ornithine decarboxylase, but to a lower level than that produced by nondialyzed sera. Insulin (0.5--10 microgram/ml) stimulated ornithine decarboxylase activity in a dose-dependent manner in serum free medium. In addition, 10(-2) M-L-asparagine stimulated ornithine decarboxylase activity in serum-free medium.
在6至17日龄鸡胚的视叶、大脑半球和中脑-间脑中观察到鸟氨酸脱羧酶活性水平随年龄增长而下降。在鸡胚脑的解离细胞培养物中，也观察到鸟氨酸脱羧酶活性随培养时间呈现类似的下降模式。通过更换培养基可刺激解离脑细胞培养物中的鸟氨酸脱羧酶活性。更换培养基后12小时显示出最大刺激效果。尽管无血清培养基对鸟氨酸脱羧酶活性有轻微刺激作用，但培养基中血清的存在是主要刺激因素。胎牛血清和热灭活胎牛血清均对鸟氨酸脱羧酶活性产生剂量依赖性刺激。透析后的胎牛血清可刺激鸟氨酸脱羧酶活性，但刺激水平低于未透析血清。胰岛素（0.5至10微克/毫升）在无血清培养基中以剂量依赖性方式刺激鸟氨酸脱羧酶活性。此外，10⁻²M - L - 天冬酰胺在无血清培养基中刺激鸟氨酸脱羧酶活性。
