通过φX174型机制指导单链到双链转化的ColE1 DNA序列的鉴定。
Identification of ColE1 DNA sequences that direct single strand-to-double strand conversion by a phi X174 type mechanism.
作者信息
Nomura N, Low R L, Ray D S
出版信息
Proc Natl Acad Sci U S A. 1982 May;79(10):3153-7. doi: 10.1073/pnas.79.10.3153.
Abstract
A DNA single-strand initiation sequence, named rriA (called rri-1 previously), was detected in the origin region (Hae II fragment E) of the ColE1 plasmid [Nomura, N. & Ray, D. S. (1980) Proc. Natl. Acad. Sci. USA 77, 6566-6570]. Another site, called rriB, has been found on the opposite strand of Hae II fragment C. Both rriA and rriB (i) direct conversion of chimeric M13 phage single-stranded DNA to parental replicative form DNA in vivo by a rifampicin-resistant mechanism that is dependent on the dnaG and dnaB gene products, (ii) provide effector sites of dATP hydrolysis by primosomal protein n', and (iii) require the same primosomal proteins as phi X174 DNA for directing the in vitro conversion that rriA is the DNA sequence that determines the mechanism of lagging strand synthesis of ColE1 DNA and that the mechanism of discontinuous synthesis involves the primosomal proteins utilized in the in vitro conversion of phi X174 single strands to the double-stranded replicative form.
摘要
在ColE1质粒的复制起点区域（Hae II片段E）中检测到一个名为rriA（以前称为rri - 1）的DNA单链起始序列[野村，N.和雷，D. S.（1980年）《美国国家科学院院刊》77，6566 - 6570]。在Hae II片段C的互补链上发现了另一个位点，称为rriB。rriA和rriB均（i）通过一种依赖于dnaG和dnaB基因产物的利福平抗性机制，在体内将嵌合M13噬菌体单链DNA转化为亲本复制型DNA，（ii）为引发体蛋白n'提供dATP水解的效应位点，并且（iii）在指导体外转化时需要与phi X174 DNA相同的引发体蛋白，即rriA是决定ColE1 DNA滞后链合成机制的DNA序列，并且不连续合成机制涉及在体外将phi X174单链转化为双链复制型时所利用的引发体蛋白。
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本文引用的文献
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Unique primed start of phage phi X174 DNA replication and mobility of the primosome in a direction opposite chain synthesis.噬菌体φX174 DNA复制独特的引发起始以及引发体沿与链合成相反方向的移动。
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