一对HLA单倍型相同的嵌合双胞胎家族中的混合淋巴细胞培养（MLC）和慢性髓性白血病（CML）研究

MLC and CML studies in the family of a pair of HLA haploidentical chimeric twins.

作者信息

Thomsen M, Hansen H E, Dickmeiss E

出版信息

Scand J Immunol. 1977;6(5):523-8. doi: 10.1111/j.1365-3083.1977.tb02115.x.

DOI:10.1111/j.1365-3083.1977.tb02115.x

Abstract

A pair of dizygotic twins of opposite sex were incidentally found to be erythrocyte chimeras. The chimerism was found to include the lymphocyte population as well, since the twins differed for the maternal while sharing one paternal HLA haplotype. In addition to lymphocytes carrying antigens controlled by the inherited maternal haplotype, each of the twins had a small fraction--about 15%--of lymphocytes of the other twin's type. Both twins are healthy and have normal children. Investigations of the family by mixed lymphocyte culture (MLC) and cell-mediated lympholysis (CML) tests showed that the two haplotypes by which the twins differed carried different HLA-D determinants; even so the lymphocytes of each twin were completely nonresponsive in MLC and CML to those of the other twin. No 'blocking' factors could be demonstrated in the serum of the twins. These results support Burnet's theory of clonal selection in fetal life.

摘要

一对异性双卵双胞胎被偶然发现是红细胞嵌合体。由于这对双胞胎在母系HLA单倍型上不同而共享一个父系HLA单倍型，所以发现这种嵌合现象也包括淋巴细胞群体。除了携带由遗传的母系单倍型控制的抗原的淋巴细胞外，每个双胞胎都有一小部分（约15%）另一个双胞胎类型的淋巴细胞。这对双胞胎都很健康，并且都有正常的孩子。通过混合淋巴细胞培养（MLC）和细胞介导的淋巴细胞溶解（CML）试验对该家族进行的调查表明，这对双胞胎不同的两个单倍型携带不同的HLA - D决定簇；即便如此，每个双胞胎的淋巴细胞在MLC和CML中对另一个双胞胎的淋巴细胞完全无反应。在双胞胎的血清中未发现“阻断”因子。这些结果支持了伯内特的胎儿期克隆选择理论。